Individual maps were constructed to compare the activations within each participant for the contrasts“unpaired milkshake cue vs unpaired tasteless solution cue” and“milkshake receipt vs tasteless solution receipt,” which were then regressed against total YFAS scores using SPM5. To detect group differences, 2 second-level 2 2 analyses of variance were conducted: (high FA group vs low FA group) (milkshake receipt vs tasteless solution receipt) and (high FA group vs low FA group) (unpaired milkshake cue vs unpaired tasteless solution cue). The T-map threshold was set at P uncorrected = .001 and a 3-voxel cluster size. We performed small volume correction analyses using peaks with the highest volumes (millimeters cubed) and z values identified previously in the cue-induced craving and drug administration literature9,34- 35 as well as in food cue/food administration studies.15,36- 37 To test our hypothesis that participants exhibiting more FA symptoms would demonstrate greater activation in response to food cues, search volumes were restricted within a 10-mm radius of reference coordinates in the OFC (42, 46,−16;−8, 60,−14), caudate (9, 0, 21), amygdala (−12,−10,−16), ACC (−10, 24, 30;−4, 30, 16), DLPFC (−30, 36, 42), thalamus (−7,−26, 9), midbrain (−12,−20,−22; 3,−28,−13), and insula (36, 12, 2). To test our hypothesis that during consumption of a highly palatable food, the high vs low FA group would demonstrate less activation in reward-related brain regions, search volumes were restricted within a 10-mm radius of reference coordinates in the OFC (±42, 46,−16;±41, 34,−19;±8, 60,−14) and caudate (±9, 0, 21;±2,−9, 34). Predicted activations were considered to be significant at P < .05 after correcting for multiple comparisons (false discovery rate corrected) across the voxels within the a priori–defined small volumes. Bonferroni corrections were then used to correct for the number of regions of interest tested. Because Dreher et al38 reported that women in the midfollicular phase (4-8 days after first period) show greater response in reward regions compared with those in the luteal phase, we attempted to run the scans for all women during the same period of the menstrual cycle. However, because of scheduling difficulties, 2 participants were scanned during the midfollicular phase. When these individuals were excluded, the relations between YFAS score and blood oxygen level–dependent responses to food intake and anticipated intake remained significant.