0
We're unable to sign you in at this time. Please try again in a few minutes.
Retry
We were able to sign you in, but your subscription(s) could not be found. Please try again in a few minutes.
Retry
There may be a problem with your account. Please contact the AMA Service Center to resolve this issue.
Contact the AMA Service Center:
Telephone: 1 (800) 262-2350 or 1 (312) 670-7827  *   Email: subscriptions@jamanetwork.com
Error Message ......
Original Investigation |

Assessment of Plasma C-Reactive Protein as a Biomarker of Posttraumatic Stress Disorder Risk FREE

Satish A. Eraly, MD, PhD1,2; Caroline M. Nievergelt, PhD2,3,4; Adam X. Maihofer, MS4; Donald A. Barkauskas, PhD5; Nilima Biswas, PhD1; Agorastos Agorastos, MD6; Daniel T. O’Connor, MD1,2; Dewleen G. Baker, MD2,3,4 ; for the Marine Resiliency Study Team
[+] Author Affiliations
1Department of Medicine, University of California, San Diego (UCSD)
2Veterans Affairs (VA) San Diego Healthcare System, San Diego, California
3VA Center of Excellence for Stress and Mental Health, San Diego, California
4Department of Psychiatry, UCSD, La Jolla
5Department of Preventive Medicine, University of Southern California, Los Angeles
6Department of Psychiatry and Psychotherapy, University Medical Center Hamburg-Eppendorf, Hamburg, Germany
JAMA Psychiatry. 2014;71(4):423-431. doi:10.1001/jamapsychiatry.2013.4374.
Text Size: A A A
Published online

Importance  Posttraumatic stress disorder (PTSD) has been associated in cross-sectional studies with peripheral inflammation. It is not known whether this observed association is the result of PTSD predisposing to inflammation (as sometimes postulated) or to inflammation predisposing to PTSD.

Objective  To determine whether plasma concentration of the inflammatory marker C-reactive protein (CRP) helps predict PTSD symptoms.

Design, Setting, and Participants  The Marine Resiliency Study, a prospective study of approximately 2600 war zone–deployed Marines, evaluated PTSD symptoms and various physiological and psychological parameters before deployment and at approximately 3 and 6 months following a 7-month deployment. Participants were recruited from 4 all-male infantry battalions imminently deploying to a war zone. Participation was requested of 2978 individuals; 2610 people (87.6%) consented and 2555 (85.8%) were included in the present analysis. Postdeployment data on combat-related trauma were included for 2208 participants (86.4% of the 2555 included) and on PTSD symptoms at 3 and 6 months after deployment for 1861 (72.8%) and 1617 (63.3%) participants, respectively.

Main Outcomes and Measures  Severity of PTSD symptoms 3 months after deployment assessed by the Clinician-Administered PTSD Scale (CAPS).

Results  We determined the effects of baseline plasma CRP concentration on postdeployment CAPS using zero-inflated negative binomial regression (ZINBR), a procedure designed for distributions, such as CAPS in this study, that have an excess of zeroes in addition to being positively skewed. Adjusting for the baseline CAPS score, trauma exposure, and other relevant covariates, we found baseline plasma CRP concentration to be a highly significant overall predictor of postdeployment CAPS scores (P = .002): each 10-fold increment in CRP concentration was associated with an odds ratio of nonzero outcome (presence vs absence of any PTSD symptoms) of 1.51 (95% CI, 1.15-1.97; P = .003) and a fold increase in outcome with a nonzero value (extent of symptoms when present) of 1.06 (95% CI, 0.99-1.14; P = .09).

Conclusions and Relevance  A marker of peripheral inflammation, plasma CRP may be prospectively associated with PTSD symptom emergence, suggesting that inflammation may predispose to PTSD.

Figures in this Article

Observational studies1 largely support an association of posttraumatic stress disorder (PTSD) with increased peripheral inflammation, as discussed in a recent review of the overall evidence.2 For instance, one large cross-sectional community-based study3 found that patients with PTSD had approximately twice the odds of those without this disorder of elevation in the inflammatory marker C-reactive protein (CRP). Similarly, although some case-control studies4,5 have had negative or equivocal findings, in most such studies611 PTSD cohorts have had significantly greater plasma levels of CRP or interleukin 6, among other inflammatory markers, than did controls. This association is of prognostic significance because low-grade inflammation is likely involved in the pathophysiology of the metabolic syndrome,1214 a major cardiovascular risk factor15,16; indeed, PTSD has been found to be associated with this syndrome.1724

It is plausible that the observed association between PTSD and inflammation is due to PTSD-related stress hormone dysregulation leading to alterations in immune, and therefore inflammatory, signaling.7,2527 However, given the cross-sectional nature of the evidence at hand, it remains possible that rather than PTSD promoting inflammation, inflammation places individuals at heightened risk for developing PTSD in the setting of trauma. In other words, the direction of causality runs from inflammation to PTSD rather than from PTSD to inflammation.

Service members serving in the Iraq and Afghanistan conflicts endure substantial combat stress and consequent PTSD.28 The Marine Resiliency Study (MRS) is a prospective field study of approximately 2600 Marines and sailors deployed to Iraq or Afghanistan, during which PTSD severity and various physiological and psychological parameters were determined predeployment and postdeployment, affording an outstanding opportunity to investigate the causal relationship between inflammation and PTSD. In the present study, we determined whether baseline peripheral inflammation, assessed by plasma CRP levels in the MRS, contributes to postdeployment PTSD symptoms, assessed by scores on the Clinician-Administered PTSD Scale (CAPS), adjusting for trauma exposure and other relevant covariates.

Participants

The MRS is a prospective longitudinal study of biological and neuropsychological modulators of combat stress–related PTSD in Marines.29 Approval was received and has been maintained since August 2007 from the institutional review boards of the University of California, San Diego, Veterans Affairs San Diego Research Service, and Naval Health Research Center. Participants were recruited from 4 all-male infantry battalions that were imminently deploying to a war zone. Participation was requested of 2978 individuals, of whom 2610 (87.6%) provided written informed consent and were enrolled and given financial compensation. Assessment of the participants began on July 14, 2008, and continued through May 24, 2012. Fifty-five of the enrollees were excluded from the present analysis because they did not deploy with their cohort or withdrew before completing the predeployment visit, so that the number of participants included was 2555 (85.8%). The demographics of these individuals are summarized in Table 1.

Table Graphic Jump LocationTable 1.  Selected Baseline and Postdeployment Characteristics of Participantsa

Data were collected approximately 1 month before a 7-month deployment (baseline; visit 0) and at 1 week, 3 months, and 6 months following the deployment (visits 1, 2, and 3, respectively). Among the 2555 included participants, baseline plasma CRP concentrations were included from 2484 participants (97.2%) and baseline CAPS scores from 2533 participants (99.1%). For the other specific baseline variables used in the present statistical analyses (anthropometrics, psychometrics, and demographics; see below), the number of individuals with included data ranged from 2482 to 2548 (97.1%-99.7%). Data on deployment-related trauma were obtained at visit 1 and were included from 2208 participants (86.4%), visit 2 CAPS scores from 1861 participants (72.8%), and visit 3 CAPS scores from 1617 participants (63.3%).

Measures

The CAPS,30 a criterion standard PTSD symptom scale, was the primary outcome measure for our analyses because, as a 136-point numeric scale, it would be expected to yield greater discriminant power than the binary outcome of PTSD diagnosis. Trauma exposure occurring during combat was assessed with the Deployment Risk and Resilience Inventory Combat Exposure Scale (CES) (http://www.ptsd.va.gov/professional/assessment/te-measures/ces.asp), and exposure occurring in the aftermath of combat with the Deployment Risk and Resilience Inventory Post-battle Experiences (PBE) scale (http://www.ptsd.va.gov/professional/assessment/deployment/exposure-aftermath-battle.asp). Baseline high-sensitive CRP plasma levels were measured using an enzyme-linked immunosorbent assay (ALPCO Diagnostics). Measures for variables not included in the final regression model are described in the Supplement (eMethods).

Statistical Analysis

The association of our predictors of interest with CAPS was determined using zero-inflated negative binomial regression (ZINBR). A description of this method and the rationale for its choice are in the Supplement (eMethods). Potential confounders were selected for inclusion in regression modeling on the basis of their univariate association at a lenient significance threshold (P < .20), with both the outcome (postdeployment CAPS) and the predictor of interest (plasma CRP concentration) (determined by analysis of variance, linear regression, or ZINBR as appropriate). The values for plasma CRP concentrations were skewed and were therefore log transformed before analyses. Ordinal and binomial logistic regression were used to determine the effects of the same predictors as in the final ZINBR model (Table 2) on the categorical outcomes at visit 2 of full PTSD (as defined in the DSM-IV-R),31 partial PTSD,3234 or no PTSD. Statistical analyses were performed with either SPSS, version 20.0 (IBM) or, for ZINBR, the R statistical package (http://cran.r-project.org). All P values reported are 2-tailed.

Table Graphic Jump LocationTable 2.  Zero-Inflated Negative Binomial Regression Model of Postdeployment (Visit 2) CAPS Score in 1719 Participants
Choice of Outcomes and Model Covariates

Baseline and postdeployment values of participants for the variables included in the statistical models are listed in Table 1 along with selected additional characteristics. Posttraumatic stress disorder symptoms, assessed by CAPS scores (see the Methods section), increased significantly between the baseline and 3-month postdeployment visits used for our analysis (visits 0 and 2), and then trended back toward baseline in line with findings in a recent systematic review.35 In contrast to their scores on CAPS, participants’ scores on the Beck Anxiety Inventory and Beck Depression Inventory (Supplement [eMethods]) dropped markedly after completion of deployment (Table 1), potentially reflecting the relief experienced by service personnel on return from combat. Thus, the observed postdeployment increases in PTSD symptoms were not attributable to broad psychopathology or general psychological distress.

We included baseline CAPS scores (CAPS0) as a covariate in all statistical analyses of the outcome of visit 2 CAPS (CAPS2) so as to adjust for any differences between participants in CAPS2 that were attributable to preexisting differences in CAPS0; this also adjusted for any effects of baseline PTSD symptoms on the subsequent trajectory of the disorder. In addition to CAPS0, CES and PBE scores (determined at visit 1 immediately following deployment) were included as covariates in regression models to adjust for differences between participants in traumatic exposure during and after combat, respectively (as detailed in the Methods section). Moreover, because the 4 MRS battalions differed from one another in their war zone experiences and in the timing of their training regimen relative to the period of data collection, cohort assignment of each participant was set as a factor in regression analysis. Multiple other potential confounders were evaluated, including several previously associated with both PTSD and peripheral inflammation (eg, baseline depression, anxiety, and alcohol and tobacco use3652) and various anthropometric and demographic variables (Table 1); however, none met the criteria for inclusion in the regression models.

ZINBR of Postdeployment CAPS

In accordance with the analyses described above, our ZINBR model (described in the Methods section) of CAPS2 comprised plasma CRP concentration, CAPS0, cohort assignment, and CES and PBE scores. C-reactive protein was a highly significant overall predictor of CAPS2 in this model (P = .002 by likelihood ratio test), as was each of the other predictors (Table 2). C-reactive protein was also a highly significant predictor in the analogous linear regression model with the same covariates (P = .002); however, ZINBR is significantly superior to linear regression when modeling the outcome of CAPS (Supplement [eMethods]). We assessed all 2-way interactions with CRP; none was statistically significant. Based on analysis of the scores on CAPS subscales, the greatest effect of CRP appeared to be in the domain of hyperarousal (subscale D of CAPS; overall P <  .001), with less of an effect on numbing (subscale CN; P = .03), and even lesser effects on reexperiencing (subscale B; P = . 28) and avoidance (subscale CA; P = .57).

In the zero component of the ZINBR model, CRP was a positive predictor of CAPS2: each 1-U increment in log10 plasma CRP concentration (ie, each 10-fold increase in CRP concentration) was associated with a fold change in the approximate odds of obtaining a CAPS2 score greater than zero (ie, odds ratio [OR] of nonzero CAPS2) of 1.51 (95% CI, 1.15-1.97; P = .003) (Table 2). Stated in the context of the range of CRP concentrations in our study population, a 1-SD increase in log10 CRP (corresponding to a 3.57-fold increase in CRP concentration) was associated with an OR of 1.25 (1.08-1.45) (Figure 1A). By comparison, 1-SD increases in CAPS0, CES, and PBE were associated with ORs of 4.15 (95% CI, 3.06-5.63; P < .001), 1.39 (1.06-1.83; P = .019), and 1.43 (1.13-1.80; P = .003), respectively. Consistent with the findings obtained with CRP treated as a continuous predictor, categorization of CRP revealed a trend toward a greater OR of nonzero outcome with increasing CRP category (although there was, as expected, a loss of statistical power) (Figure 1B).

Place holder to copy figure label and caption
Figure 1.
Effects of Baseline Plasma C-Reactive Protein (CRP) Concentration and Other Predictors on Postdeployment Posttraumatic Stress Disorder (PTSD) Symptoms

A, Adjusted odds ratios (AORs) of a nonzero Clinician-Administered PTSD Scale score at visit 2 (CAPS2) associated with 1-SD increases in the indicated variables. B, Adjusted fold changes in CAPS2 associated with 1-SD increases in the indicated variables. Data in A and B are from the zero-inflated negative binomial regression (ZINBR) model summarized in Table 2. C, The AORs of nonzero CAPS2 by baseline plasma CRP concentration category (reference category, ≤1; to convert to nanomoles per liter, multiply by 9.524), as determined by ZINBR and adjusted for the same covariates as the model in Table 2. CAPS0 indicates Clinician-Administered PTSD Scale at visit 0 (baseline); CES, Combat Exposure Scale; and PBE, Post-battle Experiences. Error bars delineate 95% CIs; the y-axes use a log scale. All P values are 2-tailed.aP < .001.bP < .05.cP < .01.dP < .10.

Graphic Jump Location

Likewise, CRP was a positive predictor of CAPS2 scores in the count component of the ZINBR model (which predicts approximately the extent of the outcome when it is nonzero, as described in the Supplement [eMethods]): each 10-fold increase in CRP concentration was associated with a 1.06-fold increase in CAPS2. However, this effect was statistically significant only at the trend level (95% CI, 0.99-1.14; P = .09 [2-tailed]) (Table 2). Accounting for the ranges in values of the predictors, a 1-SD increase in log10 CRP was associated with 1.03-fold change (95% CI, 1.00-1.07) in CAPS2; 1-SD increases in CAPS0, CES, and PBE were associated with fold changes of 1.36 (1.31-1.42; P < .001), 1.11 (1.05-1.18; P = .001), and 1.20 (1.13-1.28; P < .001), respectively (Figure 1B).

Logistic Regression of Postdeployment PTSD

In addition to analysis of the continuous outcome of CAPS2, we performed logistic regression of the categorical outcome of PTSD (DSM-IV-R definition 31) at visit 2 using the same covariates as in the ZINBR model. C-reactive protein was a significant predictor in this analysis as well, albeit not to as great a degree as in the ZINBR model: each 10-fold increment in CRP concentration was associated with a PTSD OR of 1.50 (95% CI, 1.02-2.22; P = .04) (Table 3). Taking into account the ranges of predictor values, a 1-SD increase in log10 CRP was associated with a PTSD OR of 1.25; by comparison, 1-SD increases in CAPS0, CES, and PBE were associated with ORs of 2.30, 1.43, and 1.35, respectively (Figure 2A). Conversely, adjusted baseline CRP values for participants with and without PTSD at visit 2 were 1.00 and 0.76 mg/L (to convert to nanomoles per liter, multiply by 9.524), respectively (adjusted for the same covariates as the logistic regression model) (Figure 2B). C-reactive protein was similarly a significant predictor in ordinal logistic regression of the diagnostic categories of PTSD, partial PTSD,3234 or neither (P = .03) (Supplement [eResults, eTable, and eFigure]).

Table Graphic Jump LocationTable 3.  Binomial Logistic Regression of Postdeployment (Visit 2) PTSD Diagnosis in 1719 Participants
Place holder to copy figure label and caption
Figure 2.
Effects of Baseline Plasma C-Reactive Protein (CRP) Concentration and Other Predictors on Postdeployment Posttraumatic Stress Disorder (PTSD) Diagnosis

A, Adjusted odds ratios (AORs) of posttraumatic stress disorder (PTSD) at visit 2 associated with 1-SD increases in the indicated variables. Data are from the binomial logistic regression model summarized in Table 3. The y-axis uses a log scale. B, Baseline plasma CRP concentration of participants without or with PTSD at visit 2, adjusted for the same covariates as the logistic regression model in Table 3. CAPS0 indicates Clinician-Administered PTSD Scale at visit 0 (baseline); CES, Combat Exposure Scale; PBE, Post-battle Experiences. Error bars delineate 95% CIs. All P values are 2-tailed.aP < .001.bP < .05.cP < .10.

Graphic Jump Location

We also performed subgroup analyses excluding participants at various thresholds of the model variables: plasma CRP, baseline CAPS, CES, and PBE. C-reactive protein effects in these subsets were generally similar to those obtained when considering all participants, indicating that the effects are not substantially influenced by individuals at the extremes of plasma CRP, baseline PTSD symptoms, or combat exposure (data not shown). Moreover, CRP was not significantly associated with baseline CAPS or PTSD diagnosis (P = .52 and .22, respectively), indicating that CRP is not a mediator or proxy for the effects of one of these other predictors on CAPS2.

We report a significant effect of baseline CRP on postdeployment PTSD symptom emergence in Marine and Navy combatants, suggesting that higher levels of this inflammatory marker may be prospectively associated with risk for PTSD. C-reactive protein predominantly influenced the likelihood of participants demonstrating the presence vs absence of PTSD symptoms rather than the extent of symptoms when present (as indicated by its greater statistical significance in the zero model of the ZINBR vs the count and logistic regression models) and had a greater effect on the hyperarousal and numbing symptom clusters than on the other clusters. Conceivably, high CRP levels mark a state of vulnerability to developing these symptoms of PTSD, and the influences of other factors prevail in determining the severity of symptoms once they are manifested.

It is sometimes postulated7,2527 that the observed association between PTSD and peripheral inflammation is due to the former disorder predisposing to the latter, plausibly due to PTSD-induced dysregulation of the stress axis resulting in disinhibition of proinflammatory pathways. Our data raise the converse possibility—that individuals with lesser inflammation may be relatively resilient and those with greater inflammation relatively vulnerable to developing PTSD symptoms. This supposition is also supported by the recent finding that the risk for PTSD following medical illness during military deployment is comparable to that following physical injury.53,54 However, the possibility that higher CRP levels at baseline resulted from preceding trauma cannot be excluded.

The underlying mechanism may involve the actions of inflammatory cytokines, which, in addition to their well-characterized adverse effects on metabolic and therefore cardiovascular health,1214 have adverse effects on mental health.5557 In particular, depression has long been known to be associated with increased peripheral inflammation,36,4650 with some studies37,38 suggesting that baseline inflammation may predict subsequent depression, and inflammatory cytokines are known to elicit symptoms of depression,5863 as discussed in reviews.2,56,57,64 Furthermore, peripheral inflammation has been associated6569 with impairments in memory and executive function. Notably, inflammatory cytokines have been demonstrated to significantly suppress hippocampal neurogenesis in animals70 and have been associated with low hippocampal volume in humans,71 a neuroanatomical trait that might mark vulnerability to PTSD—in studies of identical twins discordant for combat trauma exposure, twin pairs in which the combat-exposed member developed PTSD had smaller hippocampi than the other twin pairs.72,73

Nevertheless, the causal relationships between psychiatric disorders and inflammation are likely to be complex. For instance, in one recent large, prospective, population-based study, cumulative episodes of depression predicted subsequent CRP levels74 (although this effect was attenuated after controlling for body mass index and smoking, suggesting that it might be attributable in part to depression-related lifestyle changes rather than directly to the neurophysiological characteristics of depression). Moreover, with respect to PTSD, much work in animal models7577 supports the conclusion that chronic stress induces immunologic changes that culminate in a proinflammatory phenotype. Thus, inflammation may both contribute to PTSD and be a consequence of the stressors that led to the disorder.

Strengths of our study include its size, prospective design, and adjustment for multiple potential confounders. Moreover, owing to the youth of the study participants (mean age, 22.78 years) and their relative physical fitness (given the requirements for combat deployment), it is unlikely that chronic physical illness confounded the observed effects of baseline CRP on postdeployment PTSD symptoms. However, certain limitations merit discussion. The relative fitness of our cohort also limits the generalizability of our findings, as does the absence of women. In addition, whereas CRP concentrations fluctuate substantially in response to transient inflammatory states (eg, minor infections78), values in our participants were determined only once and thus may be relatively “noisy.” Moreover, use of anti-inflammatory medications, which might also have contributed to variability in CRP levels, was not ascertained in our study. However, such variability would generally be expected to bias toward the null hypothesis.

Finally, with regard to missing data, 27.2% of the participants did not have determination of CAPS2 scores. However, CRP values did not differ significantly between individuals for whom CAPS2 scores were present vs absent; conversely, CAPS2 scores were not significantly different when comparing participants for whom CRP values were present vs absent (not shown). Moreover, we found the effect size of CRP on CAPS2 or PTSD diagnosis (the CRP-associated OR or fold change) to be generally similar across subsets of participants having markedly different mean values for the various covariates in our regression models (data not shown). This suggests that even if the individuals with missing data were considerably different from the other participants with regard to CES, PBE, or baseline CAPS scores, the CRP effect sizes that would have been obtained had the data not been missing are likely to be similar to those that were in fact observed. Taken together, these results suggest that missing data might not have appreciably biased our findings concerning CRP effects on PTSD symptoms.

Our results, if validated by future studies, could have important clinical implications. If peripheral inflammation contributes to the development of PTSD, interventions to decrease inflammation, such as dietary or lifestyle modifications,7981 might ameliorate the severity of this disorder. At minimum, our findings are consistent with the adage mens sana in corpore sano: a healthy mind in a healthy body.

Submitted for Publication: July 2, 2013; final revision received September 21, 2013; accepted November 8, 2013.

Corresponding Author: Dewleen G. Baker, MD, Veterans Affairs Center of Excellence for Stress and Mental Health, 3350 La Jolla Village Dr, Ste 116A, San Diego, CA 92161 (dgbaker@ucsd.edu).

Published Online: February 26, 2014. doi:10.1001/jamapsychiatry.2013.4374.

Author Contributions: Drs Nievergelt and Baker had full access to all the data in the study and take responsibility for the integrity of the data and the accuracy of the data analysis.

Study concept and design: Eraly, Nievergelt, O’Connor, Baker.

Acquisition of data: Nievergelt, Biswas, O’Connor, Baker.

Analysis and interpretation of data: Eraly, Nievergelt, Maihofer, Barkauskas, Agorastos, Baker.

Drafting of the manuscript: Eraly, Nievergelt, Baker.

Critical revision of the manuscript for important intellectual content: All authors.

Statistical analysis: Eraly, Nievergelt, Maihofer, Barkauskas, Agorastos.

Obtained funding: Eraly, Baker.

Administrative, technical, or material support: Biswas, O’Connor.

Study supervision: Nievergelt, Baker.

Conflict of Interest Disclosures: None reported.

Funding/Support: Dr Eraly was funded by a UCSD Department of Medicine Career Award and by National Heart, Lung, and Blood Institute grant HL094728; Drs Nievergelt, O’Connor, Baker, and MRS personnel were supported by VA Health Service Research and Development Project Number SDR 09-0128, National Institutes of Health MH093500, the US Marine Corps, and the US Navy Bureau of Medicine and Surgery.

Role of the Sponsor: The sponsors had no role in the design and conduct of the study; collection, management, analysis, and interpretation of the data; preparation, review, or approval of the manuscript; and decision to submit the manuscript for publication.

Disclaimer: The content is solely the responsibility of the authors and does not necessarily represent the official views of the federal government.

Group Information:MRS coinvestigators: Mark A. Geyer, PhD (Department of Psychiatry, UCSD), Victoria B. Risbrough, PhD (VA Center of Excellence for Stress and Mental Health and Department of Psychiatry, UCSD), Gerald E. Larson, PhD (Naval Health Research Center), Brett T. Litz, PhD (Department of Psychiatry, Boston University School of Medicine, VA Boston Healthcare System), William P. Nash, MD (Boston VA Research Institute), Nicholas J. Schork, PhD (Scripps Translational Research Institute), Jennifer J. Vasterling, PhD (Department of Psychiatry, Boston University School of Medicine, VA Boston Healthcare System), Paul S. Hammer, MD (Defense Center of Excellence for Psychological Health and Traumatic Brain Injury), and Jennifer A. Webb-Murphy, PhD (Naval Center for Combat and Operational Stress Control); members of the administrative core: Anjana Patel, BS, BA, Andrew De La Rosa, BS, and Patricia Gorman, BSc, DC; and members of the MRS Team, including logistic coordinators, clinician-interviewers, and data collection staff listed in our methods article.29

Additional Contributions: Michael G. Ziegler, MD (Department of Medicine, UCSD), Deborah L. Wingard, PhD (Department of Family and Preventative Medicine, UCSD), Felix K. Yam, PharmD (School of Pharmacy, UCSD), and Florin Vaida, PhD (Department of Family and Preventative Medicine, UCSD) participated in helpful discussions. We thank the Marine and Navy Corpsmen volunteers for their military service and participation in this study.

Yehuda  R.  Post-traumatic stress disorder. N Engl J Med. 2002;346(2):108-114.
PubMed   |  Link to Article
Baker  DG, Nievergelt  CM, O’Connor  DT.  Biomarkers of PTSD: neuropeptides and immune signaling. Neuropharmacology. 2012;62(2):663-673.
PubMed   |  Link to Article
Spitzer  C, Barnow  S, Völzke  H,  et al.  Association of posttraumatic stress disorder with low-grade elevation of C-reactive protein: evidence from the general population. J Psychiatr Res. 2010;44(1):15-21.
PubMed   |  Link to Article
von Kanel  R, Begre  S, Abbas  CC, Saner  H, Gander  ML, Schmid  JP.  Inflammatory biomarkers in patients with posttraumatic stress disorder caused by myocardial infarction and the role of depressive symptoms. Neuroimmunomodulation. 2010;17(1):39-46.
PubMed   |  Link to Article
von Kanel  R, Hepp  U, Kraemer  B,  et al.  Evidence for low-grade systemic proinflammatory activity in patients with posttraumatic stress disorder . J Psychiatr Res. 2007;41(9):744-752.
PubMed   |  Link to Article
Aurer  A, Aurer-Kozelj  J, Stavljenić-Rukavina  A, Kalenić  S, Ivić-Kardum  M, Haban  V.  Inflammatory mediators in saliva of patients with rapidly progressive periodontitis during war stress induced incidence increase. Coll Antropol. 1999;23(1):117-124.
PubMed
Hoge  EA, Brandstetter  K, Moshier  S, Pollack  MH, Wong  KK, Simon  NM.  Broad spectrum of cytokine abnormalities in panic disorder and posttraumatic stress disorder. Depress Anxiety. 2009;26(5):447-455.
PubMed   |  Link to Article
Tucker  P, Jeon-Slaughter  H, Pfefferbaum  B, Khan  Q, Davis  NJ.  Emotional and biological stress measures in Katrina survivors relocated to Oklahoma. Am J Disaster Med. 2010;5(2):113-125.
PubMed   |  Link to Article
Vidovic  A, Gotovac  K, Vilibic  M,  et al.  Repeated assessments of endocrine- and immune-related changes in posttraumatic stress disorder. Neuroimmunomodulation. 2011;18(4):199-211.
PubMed   |  Link to Article
Guo  M, Liu  T, Guo  JC, Jiang  XL, Chen  F, Gao  YS.  Study on serum cytokine levels in posttraumatic stress disorder patients. Asian Pac J Trop Med. 2012;5(4):323-325.
PubMed   |  Link to Article
Maes  M, Lin  AH, Delmeire  L,  et al.  Elevated serum interleukin-6 (IL-6) and IL-6 receptor concentrations in posttraumatic stress disorder following accidental man-made traumatic events. Biol Psychiatry. 1999;45(7):833-839.
PubMed   |  Link to Article
Shoelson  SE, Herrero  L, Naaz  A.  Obesity, inflammation, and insulin resistance. Gastroenterology. 2007;132(6):2169-2180.
PubMed   |  Link to Article
Olefsky  JM, Glass  CK.  Macrophages, inflammation, and insulin resistance. Annu Rev Physiol. 2010;72:219-246.
PubMed   |  Link to Article
Glass  CK, Olefsky  JM.  Inflammation and lipid signaling in the etiology of insulin resistance. Cell Metab. 2012;15(5):635-645.
PubMed   |  Link to Article
Mottillo  S, Filion  KB, Genest  J,  et al.  The metabolic syndrome and cardiovascular risk: a systematic review and meta-analysis. J Am Coll Cardiol. 2010;56(14):1113-1132.
PubMed   |  Link to Article
Grundy  SM.  Pre-diabetes, metabolic syndrome, and cardiovascular risk. J Am Coll Cardiol. 2012;59(7):635-643.
PubMed   |  Link to Article
Agyemang  C, Goosen  S, Anujuo  K, Ogedegbe  G.  Relationship between post-traumatic stress disorder and diabetes among 105 180 asylum seekers in the Netherlands. Eur J Public Health. 2012;22(5):658-662.
PubMed   |  Link to Article
Boyko  EJ, Jacobson  IG, Smith  B,  et al.  Risk of diabetes in US military service members in relation to combat deployment and mental health. Diabetes Care. 2010;33(8):1771-1777.
PubMed   |  Link to Article
Qureshi  SU, Pyne  JM, Magruder  KM, Schulz  PE, Kunik  ME.  The link between post-traumatic stress disorder and physical comorbidities: a systematic review. Psychiatr Q. 2009;80(2):87-97.
PubMed   |  Link to Article
Jin  H, Lanouette  NM, Mudaliar  S,  et al.  Association of posttraumatic stress disorder with increased prevalence of metabolic syndrome. J Clin Psychopharmacol. 2009;29(3):210-215.
PubMed   |  Link to Article
Heppner  PS, Crawford  EF, Haji  UA,  et al.  The association of posttraumatic stress disorder and metabolic syndrome: a study of increased health risk in veterans. BMC Med. 2009;7(1):1. doi:10.1186/1741-7015-7-1.
PubMed   |  Link to Article
Violanti  JM, Fekedulegn  D, Hartley  TA,  et al.  Police trauma and cardiovascular disease: association between PTSD symptoms and metabolic syndrome. Int J Emerg Ment Health. 2006;8(4):227-237.
PubMed
Goodwin  RD, Davidson  JR.  Self-reported diabetes and posttraumatic stress disorder among adults in the community. Prev Med. 2005;40(5):570-574.
PubMed   |  Link to Article
Heppner  PS, Lohr  JB, Kash  TP, Jin  H, Wang  H, Baker  DG.  Metabolic syndrome: relative risk associated with post-traumatic stress disorder (PTSD) severity and antipsychotic medication use. Psychosomatics. 2012;53(6):550-558.
PubMed   |  Link to Article
Rohleder  N, Karl  A.  Role of endocrine and inflammatory alterations in comorbid somatic diseases of post-traumatic stress disorder. Minerva Endocrinol. 2006;31(4):273-288.
PubMed
Gill  JM, Saligan  L, Woods  S, Page  G.  PTSD is associated with an excess of inflammatory immune activities. Perspect Psychiatr Care. 2009;45(4):262-277.
PubMed   |  Link to Article
Jiang  JX.  Posttraumatic stress and immune dissonance. Chin J Traumatol. 2008;11(4):203-208.
PubMed
Seal  KH, Bertenthal  D, Miner  CR, Sen  S, Marmar  C.  Bringing the war back home: mental health disorders among 103,788 US veterans returning from Iraq and Afghanistan seen at Department of Veterans Affairs facilities. Arch Intern Med. 2007;167(5):476-482.
PubMed   |  Link to Article
Baker  DG, Nash  WP, Litz  BT,  et al.  Predictors of risk and resilience for posttraumatic stress disorder among ground combat Marines: methods of the Marine Resiliency Study. Prev Chronic Dis. 2012;9:E97. doi:10.5888/pcd9.110134.
PubMed
Blake  DD, Weathers  FW, Nagy  LM,  et al.  The development of a Clinician-Administered PTSD Scale. J Trauma Stress. 1995;8(1):75-90.
PubMed   |  Link to Article
American Psychiatric Association. Diagnostic and Statistical Manual of Mental Disorders. 4th ed, text revision. Washington, DC: American Psychiatric Association; 2000.
Schnyder  U, Moergeli  H, Klaghofer  R, Buddeberg  C.  Incidence and prediction of posttraumatic stress disorder symptoms in severely injured accident victims. Am J Psychiatry. 2001;158(4):594-599.
PubMed   |  Link to Article
Pietrzak  RH, Goldstein  MB, Malley  JC, Johnson  DC, Southwick  SM.  Subsyndromal posttraumatic stress disorder is associated with health and psychosocial difficulties in veterans of Operations Enduring Freedom and Iraqi Freedom. Depress Anxiety. 2009;26(8):739-744.
PubMed   |  Link to Article
Adams  RE, Boscarino  JA, Galea  S.  Alcohol use, mental health status and psychological well-being 2 years after the World Trade Center attacks in New York City. Am J Drug Alcohol Abuse. 2006;32(2):203-224.
PubMed   |  Link to Article
Santiago  PN, Ursano  RJ, Gray  CL,  et al.  A systematic review of PTSD prevalence and trajectories in DSM-5 defined trauma exposed populations: intentional and non-intentional traumatic events. PLoS One. 2013;8(4):e59236. doi:10.1371/journal.pone.0059236.
PubMed   |  Link to Article
Duivis  HE, de Jonge  P, Penninx  BW, Na  BY, Cohen  BE, Whooley  MA.  Depressive symptoms, health behaviors, and subsequent inflammation in patients with coronary heart disease: prospective findings from the Heart and Soul Study. Am J Psychiatry. 2011;168(9):913-920.
PubMed   |  Link to Article
Wium-Andersen  MK, Orsted  DD, Nielsen  SF, Nordestgaard  BG.  Elevated C-reactive protein levels, psychological distress, and depression in 73 131 individuals. JAMA Psychiatry. 2013;70(2):176-184.
PubMed   |  Link to Article
Gimeno  D, Kivimaki  M, Brunner  EJ,  et al.  Associations of C-reactive protein and interleukin-6 with cognitive symptoms of depression: 12-year follow-up of the Whitehall II study. Psychol Med. 2009;39(3):413-423.
PubMed   |  Link to Article
Whitfield  JB, Heath  AC, Madden  PA, Pergadia  ML, Montgomery  GW, Martin  NG.  Metabolic and biochemical effects of low-to-moderate alcohol consumption. Alcohol Clin Exp Res. 2013;37(4):575-586.
PubMed   |  Link to Article
Alkerwi  A, Boutsen  M, Vaillant  M,  et al.  Alcohol consumption and the prevalence of metabolic syndrome: a meta-analysis of observational studies. Atherosclerosis. 2009;204(2):624-635.
PubMed   |  Link to Article
Pai  JK, Hankinson  SE, Thadhani  R, Rifai  N, Pischon  T, Rimm  EB.  Moderate alcohol consumption and lower levels of inflammatory markers in US men and women. Atherosclerosis. 2006;186(1):113-120.
PubMed   |  Link to Article
Deverts  DJ, Cohen  S, Kalra  P, Matthews  KA.  The prospective association of socioeconomic status with C-reactive protein levels in the CARDIA study. Brain Behav Immun. 2012;26(7):1128-1135.
PubMed   |  Link to Article
Yanbaeva  DG, Dentener  MA, Creutzberg  EC, Wesseling  G, Wouters  EF.  Systemic effects of smoking. Chest. 2007;131(5):1557-1566.
PubMed   |  Link to Article
Fu  SS, McFall  M, Saxon  AJ,  et al.  Post-traumatic stress disorder and smoking: a systematic review. Nicotine Tob Res. 2007;9(11):1071-1084.
PubMed   |  Link to Article
Schäfer  I, Najavits  LM.  Clinical challenges in the treatment of patients with posttraumatic stress disorder and substance abuse. Curr Opin Psychiatry. 2007;20(6):614-618.
PubMed   |  Link to Article
Deverts  DJ, Cohen  S, DiLillo  VG,  et al.  Depressive symptoms, race, and circulating C-reactive protein: the Coronary Artery Risk Development in Young Adults (CARDIA) study. Psychosom Med. 2010;72(8):734-741.
PubMed   |  Link to Article
Elovainio  M, Keltikangas-Jarvinen  L, Pulkki-Raback  L,  et al.  Depressive symptoms and C-reactive protein: the Cardiovascular Risk in Young Finns Study. Psychol Med. 2006;36(6):797-805.
PubMed   |  Link to Article
Morris  AA, Zhao  L, Ahmed  Y,  et al.  Association between depression and inflammation—differences by race and sex: the META-Health study. Psychosom Med. 2011;73(6):462-468.
PubMed   |  Link to Article
Howren  MB, Lamkin  DM, Suls  J.  Associations of depression with C-reactive protein, IL-1, and IL-6: a meta-analysis. Psychosom Med. 2009;71(2):171-186.
PubMed   |  Link to Article
De Berardis  D, Campanella  D, Gambi  F,  et al.  The role of C-reactive protein in mood disorders. Int J Immunopathol Pharmacol. 2006;19(4):721-725.
PubMed
Baker  DG, Heppner  P, Afari  N,  et al.  Trauma exposure, branch of service, and physical injury in relation to mental health among US veterans returning from Iraq and Afghanistan. Mil Med. 2009;174(8):773-778.
PubMed   |  Link to Article
Rasmusson  AM, Schnurr  PP, Zukowska  Z, Scioli  E, Forman  DE.  Adaptation to extreme stress: post-traumatic stress disorder, neuropeptide Y and metabolic syndrome. Exp Biol Med. 2010;235(10):1150-1162.
PubMed   |  Link to Article
Forbes  HJ, Jones  N, Woodhead  C,  et al.  What are the effects of having an illness or injury whilst deployed on post deployment mental health? a population based record linkage study of UK army personnel who have served in Iraq or Afghanistan. BMC Psychiatry. 2012;12:178. doi:10.1186/1471-244X-12-178.
PubMed   |  Link to Article
McFarlane  AC.  Health surveillance of deployed military personnel occasionally leads to unexpected findings. BMC Med. 2012;10:126. doi:10.1186/1741-7015-1110-1126.
PubMed   |  Link to Article
Raison  CL, Borisov  AS, Majer  M,  et al Activation of central nervous system inflammatory pathways by interferon-alpha: relationship to monoamines and depression. Biol Psychiatry. 2009;65(4):296-303.
PubMed   |  Link to Article
Miller  AH, Maletic  V, Raison  CL.  Inflammation and its discontents: the role of cytokines in the pathophysiology of major depression. Biol Psychiatry. 2009;65(9):732-741.
PubMed   |  Link to Article
Reichenberg  A, Yirmiya  R, Schuld  A,  et al.  Cytokine-associated emotional and cognitive disturbances in humans. Arch Gen Psychiatry. 2001;58(5):445-452.
PubMed   |  Link to Article
Harrison  NA, Brydon  L, Walker  C, Gray  MA, Steptoe  A, Critchley  HD.  Inflammation causes mood changes through alterations in subgenual cingulate activity and mesolimbic connectivity. Biol Psychiatry. 2009;66(5):407-414.
PubMed   |  Link to Article
Eisenberger  NI, Berkman  ET, Inagaki  TK, Rameson  LT, Mashal  NM, Irwin  MR.  Inflammation-induced anhedonia: endotoxin reduces ventral striatum responses to reward. Biol Psychiatry. 2010;68(8):748-754.
PubMed   |  Link to Article
Wright  CE, Strike  PC, Brydon  L, Steptoe  A.  Acute inflammation and negative mood: mediation by cytokine activation. Brain Behav Immun. 2005;19(4):345-350.
PubMed   |  Link to Article
Miyaoka  H, Otsubo  T, Kamijima  K, Ishii  M, Onuki  M, Mitamura  K.  Depression from interferon therapy in patients with hepatitis C. Am J Psychiatry. 1999;156(7):1120.
PubMed
Musselman  DL, Lawson  DH, Gumnick  JF,  et al.  Paroxetine for the prevention of depression induced by high-dose interferon alfa. N Engl J Med. 2001;344(13):961-966.
PubMed   |  Link to Article
Valentine  AD, Meyers  CA, Kling  MA, Richelson  E, Hauser  P.  Mood and cognitive side effects of interferon-alpha therapy. Semin Oncol. 1998;25(1)(suppl 1):39-47.
PubMed
Raison  CL, Miller  AH.  Is depression an inflammatory disorder? Curr Psychiatry Rep. 2011;13(6):467-475.
PubMed   |  Link to Article
Komulainen  P, Lakka  TA, Kivipelto  M,  et al.  Serum high sensitivity C-reactive protein and cognitive function in elderly women. Age Ageing. 2007;36(4):443-448.
PubMed   |  Link to Article
Wersching  H, Duning  T, Lohmann  H,  et al.  Serum C-reactive protein is linked to cerebral microstructural integrity and cognitive function. Neurology. 2010;74(13):1022-1029.
PubMed   |  Link to Article
Cavalieri  M, Ropele  S, Petrovic  K,  et al.  Metabolic syndrome, brain magnetic resonance imaging, and cognition. Diabetes Care. 2010;33(12):2489-2495.
PubMed   |  Link to Article
Bettcher  BM, Wilheim  R, Rigby  T,  et al.  C-reactive protein is related to memory and medial temporal brain volume in older adults. Brain Behav Immun. 2012;26(1):103-108.
PubMed   |  Link to Article
Elderkin-Thompson  V, Irwin  MR, Hellemann  G, Kumar  A.  Interleukin-6 and memory functions of encoding and recall in healthy and depressed elderly adults. Am J Geriatr Psychiatry. 2012;20(9):753-763.
PubMed   |  Link to Article
Goshen  I, Kreisel  T, Ben-Menachem-Zidon  O,  et al.  Brain interleukin-1 mediates chronic stress-induced depression in mice via adrenocortical activation and hippocampal neurogenesis suppression. Mol Psychiatry. 2008;13(7):717-728.
PubMed   |  Link to Article
Frodl  T, Carballedo  A, Hughes  MM,  et al.  Reduced expression of glucocorticoid-inducible genes GILZ and SGK-1: high IL-6 levels are associated with reduced hippocampal volumes in major depressive disorder. Transl Psychiatry. 2012;2:e88. doi:10.1038/tp.2012.1014.
PubMed   |  Link to Article
Gilbertson  MW, Shenton  ME, Ciszewski  A,  et al.  Smaller hippocampal volume predicts pathologic vulnerability to psychological trauma. Nat Neurosci. 2002;5(11):1242-1247.
PubMed   |  Link to Article
Pitman  RK, Gilbertson  MW, Gurvits  TV,  et al; Harvard/VA PTSD Twin Study Investigators.  Clarifying the origin of biological abnormalities in PTSD through the study of identical twins discordant for combat exposure. Ann N Y Acad Sci. 2006;1071(11):242-254.
PubMed   |  Link to Article
Copeland  WE, Shanahan  L, Worthman  C, Angold  A, Costello  EJ.  Cumulative depression episodes predict later C-reactive protein levels: a prospective analysis. Biol Psychiatry. 2012;71(1):15-21.
PubMed   |  Link to Article
Chida  Y, Sudo  N, Sonoda  J, Hiramoto  T, Kubo  C.  Early-life psychological stress exacerbates adult mouse asthma via the hypothalamus-pituitary-adrenal axis. Am J RespirCrit Care Med. 2007;175(4):316-322.
PubMed   |  Link to Article
Wei  L, Simen  A, Mane  S, Kaffman  A.  Early life stress inhibits expression of a novel innate immune pathway in the developing hippocampus. Neuropsychopharmacology. 2012;37(2):567-580.
PubMed   |  Link to Article
Powell  ND, Bailey  MT, Mays  JW,  et al.  Repeated social defeat activates dendritic cells and enhances Toll-like receptor dependent cytokine secretion. Brain Behav Immun. 2009;23(2):225-231.
PubMed   |  Link to Article
Pepys  MB, Hirschfield  GM, Tennent  GA,  et al.  Targeting C-reactive protein for the treatment of cardiovascular disease. Nature. 2006;440(7088):1217-1221.
PubMed   |  Link to Article
Kao  PC, Shiesh  SC, Wu  TJ.  Serum C-reactive protein as a marker for wellness assessment. Ann Clin Lab Sci. 2006;36(2):163-169.
PubMed
Shen  J, Ordovas  JM.  Impact of genetic and environmental factors on hsCRP concentrations and response to therapeutic agents. Clin Chem. 2009;55(2):256-264.
PubMed   |  Link to Article
Beavers  KM, Nicklas  BJ, Shen  J,  et al.  Effects of lifestyle interventions on inflammatory markers in the metabolic syndrome. Front Biosci (Schol Ed). 2011;3(2):168-177.
PubMed   |  Link to Article

Figures

Place holder to copy figure label and caption
Figure 1.
Effects of Baseline Plasma C-Reactive Protein (CRP) Concentration and Other Predictors on Postdeployment Posttraumatic Stress Disorder (PTSD) Symptoms

A, Adjusted odds ratios (AORs) of a nonzero Clinician-Administered PTSD Scale score at visit 2 (CAPS2) associated with 1-SD increases in the indicated variables. B, Adjusted fold changes in CAPS2 associated with 1-SD increases in the indicated variables. Data in A and B are from the zero-inflated negative binomial regression (ZINBR) model summarized in Table 2. C, The AORs of nonzero CAPS2 by baseline plasma CRP concentration category (reference category, ≤1; to convert to nanomoles per liter, multiply by 9.524), as determined by ZINBR and adjusted for the same covariates as the model in Table 2. CAPS0 indicates Clinician-Administered PTSD Scale at visit 0 (baseline); CES, Combat Exposure Scale; and PBE, Post-battle Experiences. Error bars delineate 95% CIs; the y-axes use a log scale. All P values are 2-tailed.aP < .001.bP < .05.cP < .01.dP < .10.

Graphic Jump Location
Place holder to copy figure label and caption
Figure 2.
Effects of Baseline Plasma C-Reactive Protein (CRP) Concentration and Other Predictors on Postdeployment Posttraumatic Stress Disorder (PTSD) Diagnosis

A, Adjusted odds ratios (AORs) of posttraumatic stress disorder (PTSD) at visit 2 associated with 1-SD increases in the indicated variables. Data are from the binomial logistic regression model summarized in Table 3. The y-axis uses a log scale. B, Baseline plasma CRP concentration of participants without or with PTSD at visit 2, adjusted for the same covariates as the logistic regression model in Table 3. CAPS0 indicates Clinician-Administered PTSD Scale at visit 0 (baseline); CES, Combat Exposure Scale; PBE, Post-battle Experiences. Error bars delineate 95% CIs. All P values are 2-tailed.aP < .001.bP < .05.cP < .10.

Graphic Jump Location

Tables

Table Graphic Jump LocationTable 1.  Selected Baseline and Postdeployment Characteristics of Participantsa
Table Graphic Jump LocationTable 2.  Zero-Inflated Negative Binomial Regression Model of Postdeployment (Visit 2) CAPS Score in 1719 Participants
Table Graphic Jump LocationTable 3.  Binomial Logistic Regression of Postdeployment (Visit 2) PTSD Diagnosis in 1719 Participants

References

Yehuda  R.  Post-traumatic stress disorder. N Engl J Med. 2002;346(2):108-114.
PubMed   |  Link to Article
Baker  DG, Nievergelt  CM, O’Connor  DT.  Biomarkers of PTSD: neuropeptides and immune signaling. Neuropharmacology. 2012;62(2):663-673.
PubMed   |  Link to Article
Spitzer  C, Barnow  S, Völzke  H,  et al.  Association of posttraumatic stress disorder with low-grade elevation of C-reactive protein: evidence from the general population. J Psychiatr Res. 2010;44(1):15-21.
PubMed   |  Link to Article
von Kanel  R, Begre  S, Abbas  CC, Saner  H, Gander  ML, Schmid  JP.  Inflammatory biomarkers in patients with posttraumatic stress disorder caused by myocardial infarction and the role of depressive symptoms. Neuroimmunomodulation. 2010;17(1):39-46.
PubMed   |  Link to Article
von Kanel  R, Hepp  U, Kraemer  B,  et al.  Evidence for low-grade systemic proinflammatory activity in patients with posttraumatic stress disorder . J Psychiatr Res. 2007;41(9):744-752.
PubMed   |  Link to Article
Aurer  A, Aurer-Kozelj  J, Stavljenić-Rukavina  A, Kalenić  S, Ivić-Kardum  M, Haban  V.  Inflammatory mediators in saliva of patients with rapidly progressive periodontitis during war stress induced incidence increase. Coll Antropol. 1999;23(1):117-124.
PubMed
Hoge  EA, Brandstetter  K, Moshier  S, Pollack  MH, Wong  KK, Simon  NM.  Broad spectrum of cytokine abnormalities in panic disorder and posttraumatic stress disorder. Depress Anxiety. 2009;26(5):447-455.
PubMed   |  Link to Article
Tucker  P, Jeon-Slaughter  H, Pfefferbaum  B, Khan  Q, Davis  NJ.  Emotional and biological stress measures in Katrina survivors relocated to Oklahoma. Am J Disaster Med. 2010;5(2):113-125.
PubMed   |  Link to Article
Vidovic  A, Gotovac  K, Vilibic  M,  et al.  Repeated assessments of endocrine- and immune-related changes in posttraumatic stress disorder. Neuroimmunomodulation. 2011;18(4):199-211.
PubMed   |  Link to Article
Guo  M, Liu  T, Guo  JC, Jiang  XL, Chen  F, Gao  YS.  Study on serum cytokine levels in posttraumatic stress disorder patients. Asian Pac J Trop Med. 2012;5(4):323-325.
PubMed   |  Link to Article
Maes  M, Lin  AH, Delmeire  L,  et al.  Elevated serum interleukin-6 (IL-6) and IL-6 receptor concentrations in posttraumatic stress disorder following accidental man-made traumatic events. Biol Psychiatry. 1999;45(7):833-839.
PubMed   |  Link to Article
Shoelson  SE, Herrero  L, Naaz  A.  Obesity, inflammation, and insulin resistance. Gastroenterology. 2007;132(6):2169-2180.
PubMed   |  Link to Article
Olefsky  JM, Glass  CK.  Macrophages, inflammation, and insulin resistance. Annu Rev Physiol. 2010;72:219-246.
PubMed   |  Link to Article
Glass  CK, Olefsky  JM.  Inflammation and lipid signaling in the etiology of insulin resistance. Cell Metab. 2012;15(5):635-645.
PubMed   |  Link to Article
Mottillo  S, Filion  KB, Genest  J,  et al.  The metabolic syndrome and cardiovascular risk: a systematic review and meta-analysis. J Am Coll Cardiol. 2010;56(14):1113-1132.
PubMed   |  Link to Article
Grundy  SM.  Pre-diabetes, metabolic syndrome, and cardiovascular risk. J Am Coll Cardiol. 2012;59(7):635-643.
PubMed   |  Link to Article
Agyemang  C, Goosen  S, Anujuo  K, Ogedegbe  G.  Relationship between post-traumatic stress disorder and diabetes among 105 180 asylum seekers in the Netherlands. Eur J Public Health. 2012;22(5):658-662.
PubMed   |  Link to Article
Boyko  EJ, Jacobson  IG, Smith  B,  et al.  Risk of diabetes in US military service members in relation to combat deployment and mental health. Diabetes Care. 2010;33(8):1771-1777.
PubMed   |  Link to Article
Qureshi  SU, Pyne  JM, Magruder  KM, Schulz  PE, Kunik  ME.  The link between post-traumatic stress disorder and physical comorbidities: a systematic review. Psychiatr Q. 2009;80(2):87-97.
PubMed   |  Link to Article
Jin  H, Lanouette  NM, Mudaliar  S,  et al.  Association of posttraumatic stress disorder with increased prevalence of metabolic syndrome. J Clin Psychopharmacol. 2009;29(3):210-215.
PubMed   |  Link to Article
Heppner  PS, Crawford  EF, Haji  UA,  et al.  The association of posttraumatic stress disorder and metabolic syndrome: a study of increased health risk in veterans. BMC Med. 2009;7(1):1. doi:10.1186/1741-7015-7-1.
PubMed   |  Link to Article
Violanti  JM, Fekedulegn  D, Hartley  TA,  et al.  Police trauma and cardiovascular disease: association between PTSD symptoms and metabolic syndrome. Int J Emerg Ment Health. 2006;8(4):227-237.
PubMed
Goodwin  RD, Davidson  JR.  Self-reported diabetes and posttraumatic stress disorder among adults in the community. Prev Med. 2005;40(5):570-574.
PubMed   |  Link to Article
Heppner  PS, Lohr  JB, Kash  TP, Jin  H, Wang  H, Baker  DG.  Metabolic syndrome: relative risk associated with post-traumatic stress disorder (PTSD) severity and antipsychotic medication use. Psychosomatics. 2012;53(6):550-558.
PubMed   |  Link to Article
Rohleder  N, Karl  A.  Role of endocrine and inflammatory alterations in comorbid somatic diseases of post-traumatic stress disorder. Minerva Endocrinol. 2006;31(4):273-288.
PubMed
Gill  JM, Saligan  L, Woods  S, Page  G.  PTSD is associated with an excess of inflammatory immune activities. Perspect Psychiatr Care. 2009;45(4):262-277.
PubMed   |  Link to Article
Jiang  JX.  Posttraumatic stress and immune dissonance. Chin J Traumatol. 2008;11(4):203-208.
PubMed
Seal  KH, Bertenthal  D, Miner  CR, Sen  S, Marmar  C.  Bringing the war back home: mental health disorders among 103,788 US veterans returning from Iraq and Afghanistan seen at Department of Veterans Affairs facilities. Arch Intern Med. 2007;167(5):476-482.
PubMed   |  Link to Article
Baker  DG, Nash  WP, Litz  BT,  et al.  Predictors of risk and resilience for posttraumatic stress disorder among ground combat Marines: methods of the Marine Resiliency Study. Prev Chronic Dis. 2012;9:E97. doi:10.5888/pcd9.110134.
PubMed
Blake  DD, Weathers  FW, Nagy  LM,  et al.  The development of a Clinician-Administered PTSD Scale. J Trauma Stress. 1995;8(1):75-90.
PubMed   |  Link to Article
American Psychiatric Association. Diagnostic and Statistical Manual of Mental Disorders. 4th ed, text revision. Washington, DC: American Psychiatric Association; 2000.
Schnyder  U, Moergeli  H, Klaghofer  R, Buddeberg  C.  Incidence and prediction of posttraumatic stress disorder symptoms in severely injured accident victims. Am J Psychiatry. 2001;158(4):594-599.
PubMed   |  Link to Article
Pietrzak  RH, Goldstein  MB, Malley  JC, Johnson  DC, Southwick  SM.  Subsyndromal posttraumatic stress disorder is associated with health and psychosocial difficulties in veterans of Operations Enduring Freedom and Iraqi Freedom. Depress Anxiety. 2009;26(8):739-744.
PubMed   |  Link to Article
Adams  RE, Boscarino  JA, Galea  S.  Alcohol use, mental health status and psychological well-being 2 years after the World Trade Center attacks in New York City. Am J Drug Alcohol Abuse. 2006;32(2):203-224.
PubMed   |  Link to Article
Santiago  PN, Ursano  RJ, Gray  CL,  et al.  A systematic review of PTSD prevalence and trajectories in DSM-5 defined trauma exposed populations: intentional and non-intentional traumatic events. PLoS One. 2013;8(4):e59236. doi:10.1371/journal.pone.0059236.
PubMed   |  Link to Article
Duivis  HE, de Jonge  P, Penninx  BW, Na  BY, Cohen  BE, Whooley  MA.  Depressive symptoms, health behaviors, and subsequent inflammation in patients with coronary heart disease: prospective findings from the Heart and Soul Study. Am J Psychiatry. 2011;168(9):913-920.
PubMed   |  Link to Article
Wium-Andersen  MK, Orsted  DD, Nielsen  SF, Nordestgaard  BG.  Elevated C-reactive protein levels, psychological distress, and depression in 73 131 individuals. JAMA Psychiatry. 2013;70(2):176-184.
PubMed   |  Link to Article
Gimeno  D, Kivimaki  M, Brunner  EJ,  et al.  Associations of C-reactive protein and interleukin-6 with cognitive symptoms of depression: 12-year follow-up of the Whitehall II study. Psychol Med. 2009;39(3):413-423.
PubMed   |  Link to Article
Whitfield  JB, Heath  AC, Madden  PA, Pergadia  ML, Montgomery  GW, Martin  NG.  Metabolic and biochemical effects of low-to-moderate alcohol consumption. Alcohol Clin Exp Res. 2013;37(4):575-586.
PubMed   |  Link to Article
Alkerwi  A, Boutsen  M, Vaillant  M,  et al.  Alcohol consumption and the prevalence of metabolic syndrome: a meta-analysis of observational studies. Atherosclerosis. 2009;204(2):624-635.
PubMed   |  Link to Article
Pai  JK, Hankinson  SE, Thadhani  R, Rifai  N, Pischon  T, Rimm  EB.  Moderate alcohol consumption and lower levels of inflammatory markers in US men and women. Atherosclerosis. 2006;186(1):113-120.
PubMed   |  Link to Article
Deverts  DJ, Cohen  S, Kalra  P, Matthews  KA.  The prospective association of socioeconomic status with C-reactive protein levels in the CARDIA study. Brain Behav Immun. 2012;26(7):1128-1135.
PubMed   |  Link to Article
Yanbaeva  DG, Dentener  MA, Creutzberg  EC, Wesseling  G, Wouters  EF.  Systemic effects of smoking. Chest. 2007;131(5):1557-1566.
PubMed   |  Link to Article
Fu  SS, McFall  M, Saxon  AJ,  et al.  Post-traumatic stress disorder and smoking: a systematic review. Nicotine Tob Res. 2007;9(11):1071-1084.
PubMed   |  Link to Article
Schäfer  I, Najavits  LM.  Clinical challenges in the treatment of patients with posttraumatic stress disorder and substance abuse. Curr Opin Psychiatry. 2007;20(6):614-618.
PubMed   |  Link to Article
Deverts  DJ, Cohen  S, DiLillo  VG,  et al.  Depressive symptoms, race, and circulating C-reactive protein: the Coronary Artery Risk Development in Young Adults (CARDIA) study. Psychosom Med. 2010;72(8):734-741.
PubMed   |  Link to Article
Elovainio  M, Keltikangas-Jarvinen  L, Pulkki-Raback  L,  et al.  Depressive symptoms and C-reactive protein: the Cardiovascular Risk in Young Finns Study. Psychol Med. 2006;36(6):797-805.
PubMed   |  Link to Article
Morris  AA, Zhao  L, Ahmed  Y,  et al.  Association between depression and inflammation—differences by race and sex: the META-Health study. Psychosom Med. 2011;73(6):462-468.
PubMed   |  Link to Article
Howren  MB, Lamkin  DM, Suls  J.  Associations of depression with C-reactive protein, IL-1, and IL-6: a meta-analysis. Psychosom Med. 2009;71(2):171-186.
PubMed   |  Link to Article
De Berardis  D, Campanella  D, Gambi  F,  et al.  The role of C-reactive protein in mood disorders. Int J Immunopathol Pharmacol. 2006;19(4):721-725.
PubMed
Baker  DG, Heppner  P, Afari  N,  et al.  Trauma exposure, branch of service, and physical injury in relation to mental health among US veterans returning from Iraq and Afghanistan. Mil Med. 2009;174(8):773-778.
PubMed   |  Link to Article
Rasmusson  AM, Schnurr  PP, Zukowska  Z, Scioli  E, Forman  DE.  Adaptation to extreme stress: post-traumatic stress disorder, neuropeptide Y and metabolic syndrome. Exp Biol Med. 2010;235(10):1150-1162.
PubMed   |  Link to Article
Forbes  HJ, Jones  N, Woodhead  C,  et al.  What are the effects of having an illness or injury whilst deployed on post deployment mental health? a population based record linkage study of UK army personnel who have served in Iraq or Afghanistan. BMC Psychiatry. 2012;12:178. doi:10.1186/1471-244X-12-178.
PubMed   |  Link to Article
McFarlane  AC.  Health surveillance of deployed military personnel occasionally leads to unexpected findings. BMC Med. 2012;10:126. doi:10.1186/1741-7015-1110-1126.
PubMed   |  Link to Article
Raison  CL, Borisov  AS, Majer  M,  et al Activation of central nervous system inflammatory pathways by interferon-alpha: relationship to monoamines and depression. Biol Psychiatry. 2009;65(4):296-303.
PubMed   |  Link to Article
Miller  AH, Maletic  V, Raison  CL.  Inflammation and its discontents: the role of cytokines in the pathophysiology of major depression. Biol Psychiatry. 2009;65(9):732-741.
PubMed   |  Link to Article
Reichenberg  A, Yirmiya  R, Schuld  A,  et al.  Cytokine-associated emotional and cognitive disturbances in humans. Arch Gen Psychiatry. 2001;58(5):445-452.
PubMed   |  Link to Article
Harrison  NA, Brydon  L, Walker  C, Gray  MA, Steptoe  A, Critchley  HD.  Inflammation causes mood changes through alterations in subgenual cingulate activity and mesolimbic connectivity. Biol Psychiatry. 2009;66(5):407-414.
PubMed   |  Link to Article
Eisenberger  NI, Berkman  ET, Inagaki  TK, Rameson  LT, Mashal  NM, Irwin  MR.  Inflammation-induced anhedonia: endotoxin reduces ventral striatum responses to reward. Biol Psychiatry. 2010;68(8):748-754.
PubMed   |  Link to Article
Wright  CE, Strike  PC, Brydon  L, Steptoe  A.  Acute inflammation and negative mood: mediation by cytokine activation. Brain Behav Immun. 2005;19(4):345-350.
PubMed   |  Link to Article
Miyaoka  H, Otsubo  T, Kamijima  K, Ishii  M, Onuki  M, Mitamura  K.  Depression from interferon therapy in patients with hepatitis C. Am J Psychiatry. 1999;156(7):1120.
PubMed
Musselman  DL, Lawson  DH, Gumnick  JF,  et al.  Paroxetine for the prevention of depression induced by high-dose interferon alfa. N Engl J Med. 2001;344(13):961-966.
PubMed   |  Link to Article
Valentine  AD, Meyers  CA, Kling  MA, Richelson  E, Hauser  P.  Mood and cognitive side effects of interferon-alpha therapy. Semin Oncol. 1998;25(1)(suppl 1):39-47.
PubMed
Raison  CL, Miller  AH.  Is depression an inflammatory disorder? Curr Psychiatry Rep. 2011;13(6):467-475.
PubMed   |  Link to Article
Komulainen  P, Lakka  TA, Kivipelto  M,  et al.  Serum high sensitivity C-reactive protein and cognitive function in elderly women. Age Ageing. 2007;36(4):443-448.
PubMed   |  Link to Article
Wersching  H, Duning  T, Lohmann  H,  et al.  Serum C-reactive protein is linked to cerebral microstructural integrity and cognitive function. Neurology. 2010;74(13):1022-1029.
PubMed   |  Link to Article
Cavalieri  M, Ropele  S, Petrovic  K,  et al.  Metabolic syndrome, brain magnetic resonance imaging, and cognition. Diabetes Care. 2010;33(12):2489-2495.
PubMed   |  Link to Article
Bettcher  BM, Wilheim  R, Rigby  T,  et al.  C-reactive protein is related to memory and medial temporal brain volume in older adults. Brain Behav Immun. 2012;26(1):103-108.
PubMed   |  Link to Article
Elderkin-Thompson  V, Irwin  MR, Hellemann  G, Kumar  A.  Interleukin-6 and memory functions of encoding and recall in healthy and depressed elderly adults. Am J Geriatr Psychiatry. 2012;20(9):753-763.
PubMed   |  Link to Article
Goshen  I, Kreisel  T, Ben-Menachem-Zidon  O,  et al.  Brain interleukin-1 mediates chronic stress-induced depression in mice via adrenocortical activation and hippocampal neurogenesis suppression. Mol Psychiatry. 2008;13(7):717-728.
PubMed   |  Link to Article
Frodl  T, Carballedo  A, Hughes  MM,  et al.  Reduced expression of glucocorticoid-inducible genes GILZ and SGK-1: high IL-6 levels are associated with reduced hippocampal volumes in major depressive disorder. Transl Psychiatry. 2012;2:e88. doi:10.1038/tp.2012.1014.
PubMed   |  Link to Article
Gilbertson  MW, Shenton  ME, Ciszewski  A,  et al.  Smaller hippocampal volume predicts pathologic vulnerability to psychological trauma. Nat Neurosci. 2002;5(11):1242-1247.
PubMed   |  Link to Article
Pitman  RK, Gilbertson  MW, Gurvits  TV,  et al; Harvard/VA PTSD Twin Study Investigators.  Clarifying the origin of biological abnormalities in PTSD through the study of identical twins discordant for combat exposure. Ann N Y Acad Sci. 2006;1071(11):242-254.
PubMed   |  Link to Article
Copeland  WE, Shanahan  L, Worthman  C, Angold  A, Costello  EJ.  Cumulative depression episodes predict later C-reactive protein levels: a prospective analysis. Biol Psychiatry. 2012;71(1):15-21.
PubMed   |  Link to Article
Chida  Y, Sudo  N, Sonoda  J, Hiramoto  T, Kubo  C.  Early-life psychological stress exacerbates adult mouse asthma via the hypothalamus-pituitary-adrenal axis. Am J RespirCrit Care Med. 2007;175(4):316-322.
PubMed   |  Link to Article
Wei  L, Simen  A, Mane  S, Kaffman  A.  Early life stress inhibits expression of a novel innate immune pathway in the developing hippocampus. Neuropsychopharmacology. 2012;37(2):567-580.
PubMed   |  Link to Article
Powell  ND, Bailey  MT, Mays  JW,  et al.  Repeated social defeat activates dendritic cells and enhances Toll-like receptor dependent cytokine secretion. Brain Behav Immun. 2009;23(2):225-231.
PubMed   |  Link to Article
Pepys  MB, Hirschfield  GM, Tennent  GA,  et al.  Targeting C-reactive protein for the treatment of cardiovascular disease. Nature. 2006;440(7088):1217-1221.
PubMed   |  Link to Article
Kao  PC, Shiesh  SC, Wu  TJ.  Serum C-reactive protein as a marker for wellness assessment. Ann Clin Lab Sci. 2006;36(2):163-169.
PubMed
Shen  J, Ordovas  JM.  Impact of genetic and environmental factors on hsCRP concentrations and response to therapeutic agents. Clin Chem. 2009;55(2):256-264.
PubMed   |  Link to Article
Beavers  KM, Nicklas  BJ, Shen  J,  et al.  Effects of lifestyle interventions on inflammatory markers in the metabolic syndrome. Front Biosci (Schol Ed). 2011;3(2):168-177.
PubMed   |  Link to Article

Correspondence

CME
Also Meets CME requirements for:
Browse CME for all U.S. States
Accreditation Information
The American Medical Association is accredited by the Accreditation Council for Continuing Medical Education to provide continuing medical education for physicians. The AMA designates this journal-based CME activity for a maximum of 1 AMA PRA Category 1 CreditTM per course. Physicians should claim only the credit commensurate with the extent of their participation in the activity. Physicians who complete the CME course and score at least 80% correct on the quiz are eligible for AMA PRA Category 1 CreditTM.
Note: You must get at least of the answers correct to pass this quiz.
Please click the checkbox indicating that you have read the full article in order to submit your answers.
Your answers have been saved for later.
You have not filled in all the answers to complete this quiz
The following questions were not answered:
Sorry, you have unsuccessfully completed this CME quiz with a score of
The following questions were not answered correctly:
Commitment to Change (optional):
Indicate what change(s) you will implement in your practice, if any, based on this CME course.
Your quiz results:
The filled radio buttons indicate your responses. The preferred responses are highlighted
For CME Course: A Proposed Model for Initial Assessment and Management of Acute Heart Failure Syndromes
Indicate what changes(s) you will implement in your practice, if any, based on this CME course.
Submit a Comment

Multimedia

Supplement.

eMethods. Zero-inflated negative binomial regression and evaluation measures

eResults. Ordinal logistic regression

eReferences.

eFigure. Ordinal logistic regression of PTSD, partial PTSD, or neither at visit 2

eTable. Ordinal logistic regression of PTSD, partial PTSD, or neither at visit 2

Supplemental Content

Some tools below are only available to our subscribers or users with an online account.

1,893 Views
10 Citations

Related Content

Customize your page view by dragging & repositioning the boxes below.

Articles Related By Topic
Related Collections
PubMed Articles
×