Candidate arrays were prepared on nylon membranes containing, but not limited to, dopamine receptors (eg, D1, D2, D4, D5, and DAT), G-protein subunits (iα1, iα2, αs, αz, αq, αo, β, γ1, and γ2), transcription factors (CREB, CREβ2, CREM, junB, and juD c-fos), glutamate receptor mRNAs (AMPA [GluR1-4], kainite [GluR5-7], and N-methyl-D-aspartate receptor 1 [NMDA R1]), and synaptic proteins (α-synuclein, synaptophysin 1 and 2, synaptobrevin, synaptobrevin2, synaptogyrin 1a and 3, synaptic vesicle–associated protein [SNAP]23 and 25, postsynaptic density 95, and synaptotagmin VII). Inserts were amplified in 96-well plates using polymerase chain reaction analysis with M13 forward and reverse primers under the following conditions: 95°C for 5 minutes(1 cycle); 95°C for 30 seconds, 52°C for 45 seconds, and 72°C for 2 minutes (40 cycles of this combination); and 72°C for 10 minutes(1 cycle). After polymerase chain reaction analysis, aliquots underwent electrophoresis on a 1% agarose gel (1× Tris-borate–EDTA pH 8.0 and 0.05% ethidium bromide) at 5 V/cm, and the polymerase chain reaction band size was verified. Gel images were captured by means of a digital camera and archived on a computer. We spotted 250 ng of each amplified insert on a net nylon transfer membrane(HyBond XL; Amersham Pharmacia Biotech, Minneapolis, Minn) using a 96-well dot-blot apparatus (Minifold I; Schleicher & Schuell, Inc, Baltimore, Md). The DNA was crosslinked to the membrane by means of UV radiation.