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Original Article |

Decreased Thalamic Expression of the Homeobox Gene DLX1 in Psychosis FREE

Marjan Kromkamp, MD, PhD; Harry B. M. Uylings, PhD; Marten P. Smidt, PhD; Anita J. C. G. M. Hellemons, BSc; J. Peter H. Burbach, PhD; René S. Kahn, MD, PhD
[+] Author Affiliations

From the Departments of Pharmacology and Anatomy (Drs Kromkamp, Smidt, and Burbach and Ms Hellemons) and Psychiatry (Drs Kromkamp and Kahn), Rudolf Magnus Institute for Neurosciences, University Medical Center, Utrecht, the Netherlands; Netherlands Institute for Brain Research, Graduate School Neurosciences Amsterdam, KNAW (Royal Netherlands Academy of Arts and Sciences) (Dr Uylings); Department of Anatomy, VU (Free University) University Medical Center, Amsterdam (Dr Uylings).


Arch Gen Psychiatry. 2003;60(9):869-874. doi:10.1001/archpsyc.60.9.869.
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Context  A shared vulnerability to develop psychosis can be related to abnormalities in thalamic circuits in schizophrenia and bipolar disorder and could be a genetic link between these disorders. Homeobox genes involved in development and differentiation of the brain could play an important role in these disorders.

Objective  To determine whether patients with schizophrenia and bipolar disorder have different thalamic expression patterns of 2 homeobox genes, DLX1 and SHOX2 (alias OG12X or SHOT) compared with psychiatric and nonpsychiatric control subjects.

Design  Postmortem sections containing the thalamic mediodorsal nucleus were subjected to in situ hybridization with mouse Dlx1 and human SHOX2 RNA probes. The number of both DLX1- and SHOX2-positive neurons relative to Nissl-stained neurons was estimated in systematic randomly sampled volume probes.

Patients  Fifteen patients with schizophrenia, 15 with bipolar disorder with or without history of psychosis, 15 with major depressive disorder, and 15 nonpsychiatric controls from the Stanley Foundation Brain Bank.

Main Outcome Measure  Relative numbers of DLX1- and SHOX2-positive neurons in patients with schizophrenia and bipolar disorder with history of psychosis compared with psychiatric and nonpsychiatric controls.

Results  Patients with a history of psychosis showed significantly decreased relative numbers of DLX1-positive neurons compared with patients without history of psychosis and nonpsychiatric controls (P = .02), whereas no differences could be found in relative numbers of SHOX2-positive neurons (P>.15). Results were obtained blind to diagnosis, symptoms, or any other variable except hemisphere.

Conclusion  Decreased thalamic expression of DLX1 in schizophrenia and bipolar disorder with psychosis suggests shared genetic deficits in expression of this homeobox gene.

Figures in this Article

THE THALAMUS has been implicated in the pathogenesis of schizophrenia (SZ) as it plays an important role in sensory gating, a process disturbed in psychosis.1 Thalamic abnormalities found in SZ consist of decreased volume and total number of neurons of the thalamus, specifically the mediodorsal and anteroventral nucleus, in the absence of gliosis, as shown in several postmortem studies.25 This decrease in thalamic volume in SZ has been corroborated by neuroimaging studies, with an accompanying decrease in metabolism and N-acetylaspartate levels.6,7 Decreased thalamic volume has also been found in imaging studies in siblings of schizophrenia patients,810 suggesting that this anomaly is possibly related to genetic defects that could induce a susceptibility to SZ.

However, it is not clear whether the thalamic abnormalities found in SZ are specific to this disorder. Thalamic abnormalities have also been reported in neuroimaging studies in patients with chronic bipolar disorder (BPD), albeit not consistently.1118 Only 1 postmortem study has investigated volume and cell numbers of the thalamus in patients with BPD, reporting no significant differences in comparison with the schizophrenic control subjects.19 Other postmortem studies in BPD show no increase in gliosis, suggesting that brain abnormalities present in BPD are, like those in SZ, not the result of a neurodegenerative process but of a possible neurodevelopmental etiology.11

Although not all brain abnormalities in SZ can be found in BPD and vice versa,20,21 similar findings in some postmortem and imaging studies occur in both SZ and BPD and are accompanied by an overlap in epidemiology,22 symptomatology,23 and biology, especially when psychotic BPD is included.2326 Thus, a genetic link between both disorders could be the shared vulnerability to develop psychosis. This could be the result of shared gene polymorphisms or mutations in both disorders, contributing to the molecular basis of psychosis by affecting brain growth and development (eg, of the thalamus, in both patient populations).11,27

The specification of neuronal phenotypes and neuronal connectivity relies largely on gene expression programs in progenitor cells. The homeobox (or homeodomain) family comprises one of the largest classes of transcription factors and is instrumental in cell-specific gene expression.28,29 Homeobox genes have restricted expression patterns during development of the embryonic brain, and some persist during adult life.30 Genetic studies in mice and humans have demonstrated that homeobox genes can initiate and modulate cascades of gene expression that define embryonic development and differentiation.31

One of the few homeobox genes that is expressed mainly in the thalamus in the adult rat is Prx3.32 The human homologue, SHOX2 (also named OG12X or SHOT), is situated on human chromosome 3q22-26.33 In the adult rat the expression is restricted to a number of thalamic nuclei, including the mediodorsal nucleus (MD), the superior and inferior colliculus, and pontine reticular formation.32 It is not known which gene or genes are targeted by SHOX2 or Prx3, respectively. We have shown that, in accordance with rodent data, SHOX2 is also expressed in postmortem human thalamic tissue (M.K., A.J.C.G.M.H., R.S.K., M.P.S., and J.P.H.B., unpublished data, 2001).

The homeobox gene Dlx1 has been extensively investigated in rodents.34,35Dlx1 in embryo mouse brain is expressed in cells that co-express Dlx2, which are primarily late-born neuronal precursors and subsets of postmitotic cells,35 mainly in the primordia of the basal ganglia (the telencephalic medial and lateral ganglionic eminences) and ventral thalamus.34,35 Expression of Dlx1 and Dlx2 in mice seems to be required for the production, migration, and differentiation of (most of) the neocortical, hippocampal, and olfactory bulb γ-aminobutyric acid (GABA)-ergic interneurons, which are also derived from the ganglionic eminences.36,37 In humans, DLX1 and DLX2 are closely linked at chromosome 2q32.38 Although Dlx1 is not expressed in the adult mouse mediodorsal thalamic nucleus, in humans we did find expression of DLX1 messenger RNA (mRNA) in this brain structure (M.K., Ceriel H.J. Asbreuk, R.S.K., M.P.S., and J.P.H.B., unpublished data, 2001).

Both SHOX2 and Dlx1 are expressed during brain development in the primordia of the thalamus in rodents and in the human thalamus.32,35 As thalamic abnormalities found in SZ and BPD could be of possible neurodevelopmental origin with a genetic etiology, we hypothesized that possible differences in expression patterns of these genes in the MD in SZ and BPD compared with nonpsychiatric controls (NCs) might be related to these psychiatric disorders. Alternatively, we hypothesized that possible abnormalities in gene expression could be related to the presence or absence of psychosis. Therefore, we investigated the expression of SHOX2 and DLX1 in the postmortem MD of patients with SZ, BPD with and without history of psychosis, major depressive disorder (MDD) without history of psychosis, and NCs.

SUBJECTS

Five consecutive frozen 14-µm-thick coronal sections of the thalamus containing the rostral part of the MD from 15 patients with SZ, 15 with BPD with (n = 11) and without (n = 4) history of psychosis, 15 with MDD without history of psychosis, and 15 NCs were obtained from the Stanley Foundation (Bethesda, Md) Neuropathology Consortium (Table 1).39 The sections used in this study contained excellent- to good-quality mRNA, as established by the Stanley Foundation.39 All analyses were performed blind to diagnosis or any other variable, except for hemisphere. Ten subjects(1 with SZ, 3 with BPD, 1 with MDD, and 5 NCs) were excluded from analysis because of absence of the MD (n = 7), low quality of staining (n = 1), or severe damage to the tissue (n = 2).

Table Graphic Jump LocationDemographic, Clinical, and Histologic Data*
IN SITU HYBRIDIZATION AND NISSL STAINING

Digoxigenin-labeled (anti) sense RNA probes were generated according to the manufacturer's instructions (Roche Diagnostics, Basel, Switzerland). SHOX2 RNA probes were synthesized from a Hind III fragment (base pairs [bp] 1-454) of an approximately 1200-bp human cDNA clone containing part of the coding region (Semina et al,33 GenBank accession No. AF022654). As no human DLX1 cDNA clone was available, we used mouse Dlx1 cDNA, which was overall 93% identical to human DLX1 in the coding region with a stretch of 100% homology. Dlx1 RNA probes were generated from a full-length 2.8-kb mouse cDNA clone (McGuinness et al,40 GenBank accession No. NM010053). The identity of the SHOX2 and Dlx1 cDNAs was confirmed by sequencing with a DNA sequence analysis instrument (Beckman CEQ 2000; Beckman Coulter, Fullerton, Calif).

From each subject, 1 section for each probe was used in the in situ hybridization (ISH), according to Asbreuk et al,41 with a hybridization temperature of 65°C. Test sections without probe added to the hybridization mix showed no staining after completion of the ISH. Consecutive sections were Nissl stained according to standard methods.

COUNTING PROCEDURE

With the use of a microscope (Zeiss Axioskop 2) attached to a camera system (Sony PowerHAD 3 CCD color video camera; Sony Electronics Inc, Park Ridge, NJ) and image analysis software (MCID-M5; Imaging Research Inc, St Catharines, Ontario), pictures of the Nissl-stained, SHOX2- and DLX1- positive sections were taken. The MD was outlined according to the method of Morel et al.42 In systematic randomly sampled volume probes (616 × 616 × 3 µm), neurons were counted in the MD at ×200 by means of an ocular grid.43 Both Nissl and ISH sections were equally shrunk to a 3-µm thickness. In all volume probes, only neurons containing a nucleus within or in contact with the upper or right-hand border of the ocular grid were examined. Further inclusion criteria were as follows: for Nissl staining: visible single nucleolus and presence of cresyl violet–stained cytoplasm, with shape and texture typical of neurons21;for the ISH-processed sections, positive ISH staining of the cytoplasm, presence of a clear nucleus, and clear and visible outline of cell borders.

To adjust for possible differences in total number of neurons present in the MD, final estimates of positive neurons were calculated as the ratio of density number of positive neurons in the ISH section divided by the density number of neurons in the Nissl section. This resulted in the relative number of SHOX2- positive neurons/Nissl-stained neurons (SHOX2/Nissl) and of DLX1-positive neurons/Nissl-stained neurons (DLX1/Nissl), respectively. Because of the criterion of the presence of a nucleus instead of a nucleolus, neurons in the ISH were relatively easier to include, which could result in some cases in a ratio greater than 1.

STATISTICAL ANALYSIS

All sections of every fifth subject were recounted 1 to 3 days later to ensure reliability of the assessments. The intraclass correlation coefficient of these 10 recounts was 0.98 or more for Nissl, SHOX2, and DLX1 (paired-samples t test, P<.001).

Demographic, postmortem, and clinical variables were evaluated with χ2 test and 1-way analysis of variance (ANOVA) followed by Bonferroni tests when appropriate. Overall data were normally distributed, and 1-way ANOVA was used to analyze the differences in SHOX2/Nissl and DLX1/Nissl between diagnostic groups. A 2-tailed t test was used to analyze differences in SHOX2/Nissl and DLX1/Nissl between subjects with and without history of psychosis. Bonferroni-corrected α is indicated when necessary. No overall significant differences were found between hemispheres, and data from both hemispheres were pooled. Pearson correlations were determined between mRNA expression and possible confounding variables. To investigate the effect of psychosis, subjects were divided into psychosis and nonpsychosis groups, resulting in 22 subjects with psychosis (13 with SZ and 9 with BPD) and 24 subjects without psychosis (3 with BPD, 11 with MDD, and 10 NCs) for SHOX2/Nissl, and 18 with psychosis (11 with SZ and 7 with BPD) and 22 without psychosis (3 with BPD, 11 with MDD, and 8 NCs) for DLX1/Nissl.

SHOX2/NISSL

No significant differences in SHOX2/Nissl were found between subjects with SZ, BPD, MDD, and NCs (F3,42 = 0.56, P = .60) or between psychosis and nonpsychosis groups (t44 = 1.3, P>.15, data not shown).

DLX1/NISSL

One-way ANOVA showed no significant difference in DLX1/Nissl between SZ, BPD, MDD, and NC groups (F3,36 =2.05, P = .12; Figure 1A). However, the psychosis group showed a significant 35% decrease in DLX1/Nissl compared with the nonpsychosis group(t38 = 2.5, P =.02; Figure 1B). Removing the outlier in the nonpsychosis group, as shown in Figure 1B, did not affect the result (t37 = 2.4, P = .02). When the BPD group was divided into patients with (n = 7) and without (n = 3) a history of psychosis, the patients with psychosis showed a trend for decreased DLX1/Nissl (t13 = 2.3, P = .04, Bonferroni-corrected α = .05 ÷ 2 = .025), whereas the patients without psychosis showed no significant difference (t9 = 0.4, P>.70) compared with the NCs. Figure 2 shows an example of DLX1 expression in a patient with BPD with psychosis and an NC.

Place holder to copy figure label and caption
Figure 1.

Individual data for relative numbers of DLX1-positive neurons by Nissl-stained neurons by diagnosis (schizophrenia [SZ; n = 11], bipolar disorder [BPD; n = 10], major depressive disorder [MDD; n = 11], and nonpsychiatric controls [NC; n = 8]) (A) and in subjects with (PSY; n = 18) and without (NPS; n = 22; includes nonpsychiatric controls) history of psychosis (B). Horizontal lines indicate mean levels of DLX1/Nissl.

Graphic Jump Location
Place holder to copy figure label and caption
Figure 2.

Expression of DLX1 messenger RNA in the human mediodorsal thalamic nucleus detected by digoxigenin-labeled messenger RNA probe. Arrows show neurons containing blue staining, indicating presence of DLX1 messenger RNA. Bar indicates 60 µm. A, Nonpsychiatric control. B, Patient with bipolar disorder with a history of psychosis.

Graphic Jump Location

Analyses of demographic, clinical, and postmortem data are given in Table 1. No significant correlations could be found between any of the demographic, postmortem, or clinical variables and DLX1/Nissl. Presence of mood stabilizers, including lithium carbonate, or the use of typical vs atypical antipsychotics could not be related to specific levels of DLX1/Nissl.

This study investigated the expression of 2 homeobox genes by ISH in postmortem adult human brain tissue of patients with SZ, BPD, MDD, and NCs. We found decreased expression of DLX1 but not SHOX2 in the MD in patients with a history of psychosis compared with patients without history of psychosis and NCs. We were unable to identify any known demographic, histologic, or clinical variable that could possibly have induced this change in expression levels. These results are therefore taken to indicate that DLX1 expression in the human MD may play a role in the pathogenesis of psychosis.

Our results could suggest that part of the etiology in both SZ and BPD is developmental in origin, at least regarding the vulnerability to develop psychosis, or that a common degenerative process could induce loss of neuronal DLX1 expression in both SZ and BPD with psychosis. Psychotic BPD therefore may delineate a different subtype of BPD, more resembling SZ in the developmental,44 epidemiologic,22 anatomic,11,26 symptomatologic,45 and genetic23,27 aspects of psychosis.

Our study does not elucidate the type of cells that express DLX1 in the MD of the thalamus in humans, although the size and shape of the DLX1-positive cells define them as neurons. However, recent studies indicate that DLX1-positive neurons are GABAergic interneurons.37,4648 In rodents, it has been shown that Dlx1/Dlx2-positive GABAergic neurons migrate from the ganglionic eminences to the cortex, hippocampus, and olfactory bulb, but not to the thalamus during brain development.36,46 Loss of both Dlx1 and Dlx2 induces a severe reduction in cortical interneurons and lack of normal GABAergic interneurons in the hippocampus and olfactory bulb in mice.28,36,49 In the human embryonic brain, DLX1/DLX2-positive GABAergic interneurons have been shown to migrate from the ganglionic eminences to the cortex and, in contrast to the rodent and macaque, also to the MD and pulvinar of the thalamus. Thus, this thalamic stream of DLX1/DLX2-positive GABAergic neurons seems human-specific. In this study, deficits in DLX1-expressing neurons of the human MD, which are presumably derived from the ganglionic eminences, could therefore be related to deficits in cortical interneurons derived from the same progenitor cells in SZ and BPD. Several postmortem studies have shown decreased expression of markers for and displacement of cortical GABAergic interneurons in psychosis, suggesting an abnormal development of GABAergic neurons related and etiologically linked to psychosis or the vulnerability to develop it. For instance, in the prefrontal cortex, Guidotti et al25 found a decrease in reelin and glutamic acid decarboxylase 67 (GAD67) mRNA- and protein-positive neurons in patients with SZ and BPD with history of psychosis, in the same set of postmortem brains that we have used. Other studies have also shown a loss of GAD67 or the GABA membrane transporter 1 (GAT-1) mRNA and protein in the prefrontal cortex in SZ.5054 In addition, disturbed migratory processes of GABAergic interneurons in SZ have been shown with the use of nicotinamide–adenine dinucleotide phosphate–diaphorase (NADPH-d) as a marker. A significant decrease in interneurons of the dorsolateral prefrontal cortex containing NADPH-d has been found, with an accompanying significant increase of these neurons in the underlying white matter.55,56 Preliminary data suggest that this deficit is also present in BPD.57 Volk et al52(p264) already suggested that "the site of embryonic origin [of the cortical interneurons] may be associated with a greater susceptibility to altered gene expression in schizophrenia."

Another speculative explanation for the decrease in DLX1 expression but not SHOX2 expression in our study is a common degenerative pathway in psychosis. The exact function of homeobox genes that are expressed during adult life is not known, but it is thought that these genes play a role in maintenance and regulation of neuronal systems. It cannot be excluded that a yet unidentified factor affects neurons expressing DLX1 during adult life, leading to a relative progressive decrease in DLX1 expression in psychosis, although no correlation between DLX1 expression and age at onset or duration of disease could be found in our study.

In conclusion, we found decreased thalamic expression of DLX1 mRNA in patients with history of psychosis compared with patients without history of psychosis and NCs. A speculative explanation for the down-regulation of DLX1 mRNA expression in the MD of the thalamus in psychosis could be a defect in differentiation of ganglionic eminence progenitor cells or GABAergic interneurons, or a defect in migratory pathways during brain development. Down-regulation of DLX1 in patients with SZ and BPD with a history of psychosis suggests shared genetic deficits in both disorders in relation to a vulnerability to psychosis.

Corresponding author: Marjan Kromkamp, MD, PhD, Department of Pharmacology and Anatomy, Rudolf Magnus Institute for Neurosciences, University Medical Center, Utrecht Stratenum, Universiteitsweg 100, 3584 CG Utrecht, the Netherlands.

Submitted for publication January 22, 2002; final revision received February 7, 2003; accepted February 11, 2003.

This study was supported in part by NWO (Netherlands Organisation for Scientific Research) (The Hague, the Netherlands) grant 903-42-075 (Dr Smidt).

Postmortem brain specimens were donated by the Stanley Medical Research Institute's Brain Collection courtesy of Drs Michael B. Knable, E. Fuller Torrey, Maree J. Webster, Serge Weis, and Robert H. Yolken.

We thank John L. R Rubenstein, MD, PhD, for donating the mouse Dlx1 cDNA and Elena Semina, PhD, for the human SHOX2 cDNA. We are grateful to Edwin P. Martens, MSc, for his expert statistical advice.

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Morel  AMagnin  MJeanmonod  D Multiarchitectonic and stereotactic atlas of the human thalamus. J Comp Neurol. 1997;387588- 630
PubMed Link to Article
Uylings  HB About assumptions in estimation of density of neurons and glial cells. Biol Psychiatry. 2002;51840- 842
PubMed Link to Article
Torrey  EF Epidemiological comparison of schizophrenia and bipolar disorder. Schizophr Res. 1999;39101- 106
PubMed Link to Article
Perry  WMinassian  AFeifel  DBraff  DL Sensorimotor gating deficits in bipolar disorder patients with acute psychotic mania. Biol Psychiatry. 2001;50418- 424
PubMed Link to Article
Letinic  KRakic  P Telencephalic origin of human thalamic GABAergic neurons. Nat Neurosci. 2001;4931- 936
PubMed Link to Article
Letinic  KZoncu  RRakic  P Origin of GABAergic neurons in the human neocortex. Nature. 2002;417645- 649
PubMed Link to Article
Stuhmer  TPuelles  LEkker  MRubenstein  JL Expression from a Dlx gene enhancer marks adult mouse cortical GABAergic neurons. Cereb Cortex. 2002;1275- 85
PubMed Link to Article
Anderson  SAEisenstat  DDShi  LRubenstein  JL Interneuron migration from basal forebrain to neocortex: dependence on Dlx genes. Science. 1997;278474- 476
PubMed Link to Article
Akbarian  SKim  JJPotkin  SGHagman  JOTafazzoli  ABunney  WE  JrJones  EG Gene expression for glutamic acid decarboxylase is reduced without loss of neurons in prefrontal cortex of schizophrenics. Arch Gen Psychiatry. 1995;52258- 266
PubMed Link to Article
Volk  DWAustin  MCPierri  JNSampson  ARLewis  DA Decreased glutamic acid decarboxylase67 messenger RNA expression in a subset of prefrontal cortical gamma-aminobutyric acid neurons in subjects with schizophrenia. Arch Gen Psychiatry. 2000;57237- 245
PubMed Link to Article
Volk  DAustin  MPierri  JSampson  ALewis  D GABA transporter-1 mRNA in the prefrontal cortex in schizophrenia: decreased expression in a subset of neurons. Am J Psychiatry. 2001;158256- 265
PubMed Link to Article
Pierri  JNChaudry  ASWoo  TULewis  DA Alterations in chandelier neuron axon terminals in the prefrontal cortex of schizophrenic subjects. Am J Psychiatry. 1999;1561709- 1719
PubMed
Benes  FMBerretta  S GABAergic interneurons: implications for understanding schizophrenia and bipolar disorder. Neuropsychopharmacology. 2001;251- 27
PubMed Link to Article
Akbarian  SVinuela  AKim  JJPotkin  SGBunney  WE  JrJones  EG Distorted distribution of nicotinamide-adenine dinucleotide phosphate-diaphorase neurons in temporal lobe of schizophrenics implies anomalous cortical development. Arch Gen Psychiatry. 1993;50178- 187
PubMed Link to Article
Akbarian  SBunney  WE  JrPotkin  SGWigal  SBHagman  JOSandman  CAJones  EG Altered distribution of nicotinamide-adenine dinucleotide phosphate-diaphorase cells in frontal lobe of schizophrenics implies disturbances of cortical development. Arch Gen Psychiatry. 1993;50169- 177
PubMed Link to Article
Guidotti  APesold  CDavidkova  GLiu  WSRodriguez  MADavis  JMCosta  E Altered distribution of NADPH-diaphorase positive neurons in the frontal cortex of reelin-deficient schizophrenic and bipolar disorder postmortem brain[abstract 238.4].  Paper presented at: Society for Neuroscience's 31st Annual Meeting November 12, 2001 San Diego, Calif.

Figures

Place holder to copy figure label and caption
Figure 1.

Individual data for relative numbers of DLX1-positive neurons by Nissl-stained neurons by diagnosis (schizophrenia [SZ; n = 11], bipolar disorder [BPD; n = 10], major depressive disorder [MDD; n = 11], and nonpsychiatric controls [NC; n = 8]) (A) and in subjects with (PSY; n = 18) and without (NPS; n = 22; includes nonpsychiatric controls) history of psychosis (B). Horizontal lines indicate mean levels of DLX1/Nissl.

Graphic Jump Location
Place holder to copy figure label and caption
Figure 2.

Expression of DLX1 messenger RNA in the human mediodorsal thalamic nucleus detected by digoxigenin-labeled messenger RNA probe. Arrows show neurons containing blue staining, indicating presence of DLX1 messenger RNA. Bar indicates 60 µm. A, Nonpsychiatric control. B, Patient with bipolar disorder with a history of psychosis.

Graphic Jump Location

Tables

Table Graphic Jump LocationDemographic, Clinical, and Histologic Data*

References

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Morel  AMagnin  MJeanmonod  D Multiarchitectonic and stereotactic atlas of the human thalamus. J Comp Neurol. 1997;387588- 630
PubMed Link to Article
Uylings  HB About assumptions in estimation of density of neurons and glial cells. Biol Psychiatry. 2002;51840- 842
PubMed Link to Article
Torrey  EF Epidemiological comparison of schizophrenia and bipolar disorder. Schizophr Res. 1999;39101- 106
PubMed Link to Article
Perry  WMinassian  AFeifel  DBraff  DL Sensorimotor gating deficits in bipolar disorder patients with acute psychotic mania. Biol Psychiatry. 2001;50418- 424
PubMed Link to Article
Letinic  KRakic  P Telencephalic origin of human thalamic GABAergic neurons. Nat Neurosci. 2001;4931- 936
PubMed Link to Article
Letinic  KZoncu  RRakic  P Origin of GABAergic neurons in the human neocortex. Nature. 2002;417645- 649
PubMed Link to Article
Stuhmer  TPuelles  LEkker  MRubenstein  JL Expression from a Dlx gene enhancer marks adult mouse cortical GABAergic neurons. Cereb Cortex. 2002;1275- 85
PubMed Link to Article
Anderson  SAEisenstat  DDShi  LRubenstein  JL Interneuron migration from basal forebrain to neocortex: dependence on Dlx genes. Science. 1997;278474- 476
PubMed Link to Article
Akbarian  SKim  JJPotkin  SGHagman  JOTafazzoli  ABunney  WE  JrJones  EG Gene expression for glutamic acid decarboxylase is reduced without loss of neurons in prefrontal cortex of schizophrenics. Arch Gen Psychiatry. 1995;52258- 266
PubMed Link to Article
Volk  DWAustin  MCPierri  JNSampson  ARLewis  DA Decreased glutamic acid decarboxylase67 messenger RNA expression in a subset of prefrontal cortical gamma-aminobutyric acid neurons in subjects with schizophrenia. Arch Gen Psychiatry. 2000;57237- 245
PubMed Link to Article
Volk  DAustin  MPierri  JSampson  ALewis  D GABA transporter-1 mRNA in the prefrontal cortex in schizophrenia: decreased expression in a subset of neurons. Am J Psychiatry. 2001;158256- 265
PubMed Link to Article
Pierri  JNChaudry  ASWoo  TULewis  DA Alterations in chandelier neuron axon terminals in the prefrontal cortex of schizophrenic subjects. Am J Psychiatry. 1999;1561709- 1719
PubMed
Benes  FMBerretta  S GABAergic interneurons: implications for understanding schizophrenia and bipolar disorder. Neuropsychopharmacology. 2001;251- 27
PubMed Link to Article
Akbarian  SVinuela  AKim  JJPotkin  SGBunney  WE  JrJones  EG Distorted distribution of nicotinamide-adenine dinucleotide phosphate-diaphorase neurons in temporal lobe of schizophrenics implies anomalous cortical development. Arch Gen Psychiatry. 1993;50178- 187
PubMed Link to Article
Akbarian  SBunney  WE  JrPotkin  SGWigal  SBHagman  JOSandman  CAJones  EG Altered distribution of nicotinamide-adenine dinucleotide phosphate-diaphorase cells in frontal lobe of schizophrenics implies disturbances of cortical development. Arch Gen Psychiatry. 1993;50169- 177
PubMed Link to Article
Guidotti  APesold  CDavidkova  GLiu  WSRodriguez  MADavis  JMCosta  E Altered distribution of NADPH-diaphorase positive neurons in the frontal cortex of reelin-deficient schizophrenic and bipolar disorder postmortem brain[abstract 238.4].  Paper presented at: Society for Neuroscience's 31st Annual Meeting November 12, 2001 San Diego, Calif.

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