Hippocampus and Amygdala Morphology in Attention-Deficit/Hyperactivity Disorder FREE

The neural basis of attention-deficit/hyperactivity disorder (ADHD) is currently unknown. Affecting 3% to 7% of all children and adolescents,^1- 2 ADHD is defined by distractibility, hyperactivity, and impulsivity.³ Children with ADHD often also struggle with deficits in executive functioning,⁴ working⁵ and visuospatial memory,⁶ temporal processing,⁷ and difficulty tolerating delayed rewards.⁸ The hippocampus likely subserves these functions in attention and cognition, disturbances of which are among the defining hallmarks of ADHD.^1,5,9- 13 Involvement of the amygdala in the pathophysiology of ADHD^14- 15 likely contributes to the increased risk for affective disorders in children with ADHD and their family members,^11,16- 18 even in family members who themselves do not have ADHD.^19- 21 Indeed, the association of affective and ADHD symptoms is sufficiently tight that affective symptoms previously were listed as associated features of ADHD in DSM-III-R.²²

Replicated findings in anatomical magnetic resonance imaging studies of children with ADHD include reduced cerebral volumes^23- 25 and more localized reductions in volume of the prefrontal cortex (PFC),^25- 27 particularly its inferior aspect.²⁸ Connectivity of these prefrontal regions, especially the ventral medial PFC, with the hippocampus and amygdala regulates a variety of attentional, memory, and emotional processes^29- 31 implicated in the pathophysiology of ADHD. Circuits connecting the amygdala and orbitofrontal cortex (OFC) support decision making³² and reward reinforcement,³³ and disturbances of these circuits seem to cause behavioral disinhibition and impulsivity.^34- 37

We used magnetic resonance imaging to study hippocampus and amygdala morphologies in children with ADHD and age-matched healthy controls. Our a priori hypothesis was that volumes would differ across diagnostic groups.

Subjects included 114 children and adolescents aged 7 to 18 years. We recruited children who met DSM-IV criteria³ for the combined-type ADHD. Healthy controls were recruited randomly from a telemarketing list of 10 000 names, matched by zip code to subjects with ADHD. Exclusion criteria for controls included a lifetime history of ADHD, tic disorder, or obsessive-compulsive disorder or a current DSM-IV Axis I disorder. Exclusion criteria for children with ADHD included lifetime obsessive-compulsive disorder or tics or premature birth (gestation ≤36 weeks). Additional exclusion criteria for both groups included epilepsy, head trauma with loss of consciousness, lifetime substance abuse, psychotic disorder, developmental delay, or IQ less than 80, as measured by the Wechsler Intelligence Scale for Children–III,³⁸ the Wechsler Adult Intelligence Scale–III,³⁹ or the Kaufmann Brief Intelligence Test.⁴⁰ Written informed consent was obtained from all parents, and participants provided written assent.

Clinical diagnoses were established using the Schedule for Affective Disorders and Schizophrenia for School-Age Children–Present and Lifetime Version⁴¹ and a “best-estimate consensus procedure” that considered all available clinical and diagnostic information.⁴² The ADHD symptoms were further assessed by the Conners Parent and Teacher Rating scales^43- 44 and the DuPaul-Barkley ADHD rating scale^45- 46; anxiety symptoms were assessed with the Revised Children's Manifest Anxiety Scale⁴⁷; and depressive symptoms, with the Children's Depression Inventory.⁴⁸ Socioeconomic status was estimated using the Hollingshead Four-Factor Index of Social Status.⁴⁹

Subjects were predominantly right-handed (90.2% of children with ADHD, 93.7% of controls).⁵⁰ Statistical analyses included 51 children with ADHD and 63 controls of comparable age (mean [SD] age, children with ADHD, 12.3 [3.01] years; controls, 11.5 [3.04] years; t = 1.4; P = .16), socioeconomic status (mean [SD] Hollingshead index score, children with ADHD, 45.0 [13.0]; controls, 48.3 [9.9]; t = 1.5; P = .14), and IQ (mean [SD] full-scale IQ, children with ADHD, 108.3 [19.3]; controls, 114.6 [17.1]; t = −1.7; P = .08). The ADHD group contained fewer females (ADHD, 9%; controls, 21%; χ²P<.06). Thirty-five (69%) of the subjects with ADHD were taking medication: all of them were taking stimulants, 3 were taking α-agonists, and 2 were taking selective serotonin reuptake inhibitors. No controls were taking psychotropic medication. In the ADHD group, 14 (27%) had a lifetime diagnosis of depression, 3 of whom were currently depressed; 14 subjects (27%) had oppositional defiant disorder in their lifetimes, 5 currently; 8 (16%) met lifetime criteria for specific developmental disorder (eg, reading, mathematics, written expression, or motor coordination); and 6 (11%) had a lifetime diagnosis of specific phobia, 2 had a current diagnosis of specific phobia.

Head position was standardized using canthomeatal landmarks. T1-weighted, sagittal, 3-dimensional volume images were acquired using a spoiled gradient echo pulse sequence with repetition time = 24 milliseconds, echo time = 5 milliseconds, 45° flip angle, 256 × 192 matrix, 30-cm field of view, 2 excitations, section thickness = 1.2 mm, and 124 contiguous sections.

Image processing was performed on Sun Ultra 10 workstations with ANALYZE 7.5 software (Biomedical Imaging Resource, Mayo Foundation, Rochester, Minn). Operators were blind to subject characteristics and hemisphere (images were randomly flipped left to right prior to analysis). Large-scale variations in image intensity were removed,⁵¹ and images were reformatted to standardize head positioning prior to region definition.⁵² Axial sections were oriented parallel to both the anterior and posterior commissures, and sagittal sections were oriented parallel to standard midline landmarks.⁵²

Methods for defining the hippocampus and the amygdala followed previously published algorithms (Figure 1).⁵³ The rostral extent of the amygdala coincided with the most anterior section in which the anterior commissure crossed the midline. The transition between the amygdala and hippocampus was determined with a line connecting the inferior horn of the lateral ventricle with the amygdaloid sulcus or, when the sulcus was not obvious, with a straight horizontal line connecting the inferior horn of the lateral ventricle with the surface on the uncus.⁵⁴ The most posterior section was the last section in which the crus of the fornix and the fimbria of the hippocampal formation could be delineated. Intraclass correlation coefficients, calculated using 2-way random effects,⁵⁵ were 0.91 and 0.92 for the right and left hippocampus and 0.89 and 0.88 for the right and left amygdala, respectively.

An isointensity contour function was used in conjunction with manual editing to isolate the cerebrum. This whole brain volume (WBV) measure included gray and white matter, ventricular cerebrospinal fluid, cisterns, fissures, and cortical sulci. Cerebrospinal fluid was included using a connected components analysis. The WBV did not differ significantly between the diagnostic groups and was therefore used as a covariate in statistical analyses to control for scaling effects.⁵⁶

Prefrontal regions were delineated by subdividing the cerebrum into dorsal prefrontal, inferior occipital, midtemporal, orbitofrontal, premotor, parieto-occipital, subgenual, and sensorimotor regions, as described previously.⁵² Additionally, corresponding gray matter volumes were defined and calculated for the different cortical regions. Volumes of gray matter in the dorsal prefrontal cortex (DPFC) and OFC were used for further analyses. Intraclass correlation coefficients were >0.98 for WBV and all cortical subdivisions.

Surface morphologies of the hippocampus and amygdala were compared across diagnostic groups while covarying statistically for age and sex to localize the portions of each structure that contributed most to the observed differences in global volume between groups. We computed the distance from each point on the surfaces of the hippocampus and amygdala of each subject to the corresponding point on the hippocampus and amygdala of a reference subject (R.B., L. H. Staib, PhD, D. Xu, PhD, H.Z., B.S.P., unpublished data, October-November 2005):

Detecting, localizing, and interpreting the statistically significant differences between groups in these surface analyses could conceivably depend on the choice of the reference brain. Therefore, in the steps to determine point correspondences between structures of each brain, we first selected a reference subject who was demographically as representative as possible of the children studied. The brains for all remaining subjects were coregistered to this preliminary reference. The point correspondences on the surfaces of their hippocampus and amygdala were determined, and we computed distances between the corresponding points. We then selected as the final reference the brain for which all points across the surface of the hippocampus and amygdala were closest, in terms of least squares, to the average of the computed distances. The procedures for registration, determination of point correspondences, and calculation of distances from the final reference structure were repeated for all subjects. The distances were then compared across groups.

We tested our hypothesis that volumes would differ across diagnostic groups by assessing the main effect of group and the group × region interaction in a mixed-model analysis with repeated measures over a spatial domain (amygdalar and hippocampal volumes in each hemisphere). The model included the within-subjects factors “hemisphere” with 2 levels (left and right) and “region” with 2 levels (amygdala and hippocampus). Diagnosis (ADHD and control) was a between-subjects factor. Covariates included age, sex, and WBV. Beyond these independent variables, we considered all 2- and 3-way interactions of diagnosis (ADHD), sex, hemisphere, region, and age, as well as the 2-way interactions of WBV with hemisphere or region. Other variables considered in the model were handedness, socioeconomic status, medication, IQ, lifetime diagnoses of depression, oppositional defiant disorder, or specific developmental disorder (and their 2-way interaction with region); these were treated as potential confounding variables. Statistically nonsignificant terms were eliminated via backward stepwise regression, with the constraint that the model at each step had to be hierarchically well formulated (ie, all possible lower-order terms were included in the model, regardless of statistical significance).⁵⁹ To control for a trend toward a sex imbalance across diagnostic groups, the procedure was repeated for boys only (n = 42 in both groups). We considered P values <.025 statistically significant, given our testing of 2 a priori hypotheses. All P values were 2-sided. Statistical procedures were performed in SAS version 9.0 (SAS Institute Inc, Cary, NC) or Statistical Product and Service Solutions (SPSS Inc, Chicago, Ill).⁶⁰

Associations of hippocampal and amygdalar volumes with the severity of current ADHD symptoms were assessed in the ADHD group (n = 47) while controlling for WBV, age, and sex using multiple linear regression. The correlations were restricted to the ADHD group only because of the presence of insufficient symptom variance in the control group. Correlations of the amygdala and hippocampus with anxiety and depression symptoms were assessed similarly but in both diagnostic groups.

Prefrontal gray matter volumes (OFC and DPFC volumes bilaterally) were compared between children with ADHD and controls using a 2-sided t test. This comparison was not part of our a priori hypothesis testing. We report the results of this comparison merely to document the presence of anatomical abnormalities in the frontal cortices of this sample that are similar to abnormalities reported previously in other samples of individuals with ADHD.

We explored the presumed connectivity between the hippocampal, amygdalar, and PFC subregions (DPFC and OFC gray matter volumes). Correlations were controlled for WBV, age, and sex. Differences in correlation coefficients between diagnostic groups were tested using the test statistic D for comparing 2 Pearson correlations while correcting for df_i:

where Z_i is the Fisher transformation of the correlation coefficients for samples of size n_i, and df_i = n−p−1, for partial correlations with P = 3 covariates (WBV, age, and sex).

The signed Euclidean distances between points on the surfaces of the amygdala and hippocampus for each subject and corresponding points on the respective reference structures were compared statistically between groups using linear regression at each voxel on the surface while covarying for age and sex. P values were color coded at each voxel and displayed across the surface of the reference structures. To minimize type I errors, a threshold of P<.0001 was set. Similar maps were constructed for P values associated with partial correlations r of surface measures with symptom severity in the ADHD group, while covarying with sex and age.

Testing the null hypothesis at each point on a surface generally requires many statistical comparisons. Correction of P values for these comparisons is complicated by intercorrelations among the signed distances at neighboring points. We therefore used the theory of gaussian random fields (GRFs)⁶¹ to correct P values appropriately for these multiple comparisons in the presence of intercorrelated measures across voxels. The signed distances determine a t statistic at each corresponding point, which together across the surface compose a random field f. The expected value of the Euler characteristic of the random field f was used to approximate the critical point for determining locations on the surface where the t statistics differ between groups at a prespecified significance level or statistical threshold (R.B., L. H. Staib, PhD, D. Xu, PhD, H.Z., B.S.P., unpublished data, October-November 2005).⁶² Because the expected Euler characteristic was evaluated for a GRF, t statistics at each location of the brain were first converted into values from a gaussian random variable.⁶³ Thus, surface locations where the converted statistics were larger than the estimated critical point were considered statistically significant.

The test for fixed effects in a mixed model revealed a highly significant group × region interaction (F₁₁₂ = 7.96; P<.006), demonstrating a regional specificity in group differences of amygdalar and hippocampal volumes.

Post hoc assessment of the origin of this regionally specific difference between groups in volume, using a test of differences in least-square means, indicated that the hippocampus was larger bilaterally in the children with ADHD than in the controls (3384.2 mm³ vs 3164.1 mm³; t₁₁₂ = 3.35; P<.002). Amygdalar volumes did not differ significantly across diagnostic groups (ADHD, 2062.6 mm³ vs 2106.0 mm³; t₁₁₂ = −0.64; P = .53). Other significant covariates in the model were WBV (F₁₁₁ = 39.8; P<.0001), indicating the presence of significant scaling effects, and hemisphere (F₁₁₃ = 6.1; P<.02), reflecting significantly larger volumes in the right hemisphere. A group × region × hemisphere interaction was not significant (at the point of elimination, F₁₁₁ = 0.3; P = .62), indicating the absence of significant lateralizing effects across groups. The group × region × age interaction also was not significant (F₃₂₂ = 0.2; P = .70), indicating the stability of findings across the age range of children studied. The variables sex (F₁₀₉ = 0.5; P = .48) and age (F₁₀₉ = 0.7; P = .42) were conservatively retained in the final model because of the biological plausibility that these variables could influence the overall findings.

The group × region interaction remained significant (F₈₂ = 4.37; P<.04), with a larger hippocampus in boys with ADHD compared with controls (3398.8 mm³ vs 3222.6 mm³; t₈₂ = 2.23; P<.03).

Statistical maps revealed that global differences in hippocampal volume between groups arose mainly from enlargement of the anterior hippocampus in children with ADHD (Figure 2A), particularly over the anatomical subfields cornu ammonis (CA) and dentate gyrus (DG) (Figure 3). In posterior portions of the hippocampus, in contrast, smaller indented regions bilaterally suggested the presence of reduced volumes locally in underlying tissue in the ADHD group.

Several portions of the surface of the amygdala suggested the presence of locally reduced volumes in the ADHD group that were not evident in the more conventional measures of overall volume of this structure, with several clusters of voxels reaching P values <.0001 (Figure 2B). Differences in size were located primarily over the basal nucleus of the right amygdala and lateral nucleus of the left.

Gaussian random field–based corrections for multiple comparisons produced clusters of significant voxels that were similar in location to, but smaller in size than, clusters identified in uncorrected comparisons at a threshold of P<.0001 (Figures 2, 4, and 5).

In children with ADHD only, while controlling for WBV, sex, and age, a statistical trend was detected for an inverse correlation of hippocampal volume with ratings of the severity of ADHD symptoms in the right (r = −0.29; P = .06) and left (r = −0.27; P<.07) hemispheres (Figure 6). Surface analyses also suggested that symptom severity correlated inversely with the local features of hippocampus morphology, particularly in portions that were enlarged relative to controls (Figure 4).

In children with ADHD only, volumes of the left (r = 0.3; P<.07) and right (r = 0.3; P<.06) amygdala showed strong trends toward positive correlations with hyperactivity scores (Figure 6B). Supporting the validity of these trends detected for overall volumes, analyses of symptom severity with surface features exhibited large clusters of positive correlations for hyperactivity scores bilaterally (Figure 5A). Inattention scores, in contrast, correlated inversely with surface morphology mainly in the left amygdala (Figure 5B). Symptoms of anxiety and depression did not correlate significantly with amygdalar or hippocampal volumes.

The ADHD group had significantly smaller volumes of the left OFC gray matter (ADHD, 10.535 cm³ vs controls, 11.979 cm³; t = 2.24; P<.03) and a trend toward lower mean volumes of right OFC gray matter (ADHD, 10.973 cm³ vs controls, 12.033 cm³; t = 1.68; P<.09) (Table 1). Groups did not differ in volumes of DPFC gray matter.

Interregional correlation analyses revealed positive correlations in the control group (n = 56) for the right and left amygdala with OFC gray matter (right, r = 0.66; P<.001; left, r = 0.48; P<.001) (Table 2). None of these correlations were significant in the ADHD group (n = 47). The test statistic D for comparing 2 Pearson correlation coefficients confirmed significant group differences for these correlations for the left (P<.02) and right (P<.001) amygdala.

In separate assessments of the statistical model used for hypothesis testing, none of the possible confounds reached statistical significance: lifetime diagnosis of depression (F₁₁₁ = 0.3; P = .61), oppositional defiant disorder (F₁₁₁ = 0.2; P = .67), specific developmental disorder (F₁₁₁ = 1.96; P = .17), full-scale IQ (F₉₉ = 0.3; P = .62), handedness (F₁₀₉ = 1.5; P = .22), socioeconomic status (F₁₀₁ = 1.0; P = .31), medication status (F₁₁₀ = 1.7; P = .20), and stimulant medication (F₉₆ = 2.2; P = .14). In addition, verbal (right hippocampus, r = −0.10; P = .32; left hippocampus, r = −0.10; P = .35; right amygdala, r = 0.17; P = .11; left amygdala, r = 0.15; P = .16), performance (right hippocampus, r = −0.10; P = .35; left hippocampus, r = −0.19; P = .07; right amygdala, r = 0.15; P = .15; left amygdala, r = 0.07; P = .47), and full-scale IQs (right hippocampus, r = −0.10; P = .33; left hippocampus, r = −0.14; P = .16; right amygdala, r = 0.17; P = .09; left amygdala, r = 0.12; P = .22) did not correlate significantly with regional volumes of either the hippocampus or amygdala while controlling for WBV, sex, and age, further suggesting that IQ measures did not unduly influence findings of the primary analyses.

Children and adolescents with ADHD had larger hippocampal volumes than did healthy controls, primarily deriving from larger volumes of the head of the hippocampus. Larger volumes tended to accompany less severe ADHD symptoms. Although overall volumes of the amygdala did not differ between subjects with ADHD and controls, surface analyses showed that several amygdalar subregions were smaller in children with ADHD than in controls, and these same regions generally correlated significantly and positively with the severity of ADHD symptoms. Finally, interregional correlations suggested that connectivity between the amygdala and the OFC was disrupted in the ADHD group. Medication, comorbid illnesses of affective and anxiety disorders, symptoms of depression and anxiety, and group differences in IQ did not account for our findings.

Surface analyses revealed enlarged anterior-most portions of the hippocampus in the ADHD group, particularly in its dorsal and lateral aspects, corresponding respectively to the DG and CA1-CA2 subregions.^54,64 Significant inverse correlations with hyperactivity scores were localized laterally over the CA1 and CA2 subfields, and inverse correlations with inattentive symptoms were located medially, primarily over the CA3 and DG subfields. Although we cannot infer causation from these cross-sectional, correlational findings,^69- 70 the most likely explanation for the association of more prominent enlargement with fewer ADHD symptoms, particularly in the presence of overall enlargement (ie, progressively fewer symptoms that accompany an increasingly more prominent morphological abnormality relative to controls), would seem to be that the hippocampal enlargement represents a compensatory plastic hypertrophic response to the presence of ADHD symptoms. This interpretation is consistent with abundant preclinical evidence for the presence of synaptic remodeling^71- 72 and neurogenesis⁷³ within the hippocampus, which supports improved learning and memory functions in response to experiential demands.^74- 75

An enlarged anterior hippocampus could represent a localized compensatory response of neural processes to the presence of functional disturbances in these same neural systems within the anterior hippocampus, as is thought to occur in the presence of impaired neural processing.^76- 77 Alternatively, given evidence herein and elsewhere^{24,28,78- 79} for the presence of impaired structure and function of the PFC in children with ADHD, the enlarged anterior portions of the hippocampus may represent a neural compensation for disturbances in prefrontal portions of a PFC-hippocampal network. The absence of a significant contribution of age to the correlations of hippocampus morphology with the severity of symptoms could evidence an initiation of a plastic response early in the course of disease, a possibility consistent with the shorter time frames (days to weeks) in which plasticity typically manifests.⁸⁰

The anterior hippocampus encodes the spatial and temporal relationships between sensory experiences,^81- 84 which the posterior hippocampus then consolidates for storage in long-term memory.^85- 86 Working within a distributed network that includes the PFC, the encoding of temporal relationships within the hippocampus helps to define and encode the serial ordering of events,^82,87- 90 the cognitive function probably most consistently disturbed in children with ADHD.^7,91- 96 In humans, the anterior hippocampus plays a prominent role in indexing novelty, detecting change, and exploring new environments,^86,97- 99 and thus, the stimulus-seeking behaviors of children with ADHD¹⁰⁰ may engage these anterior hippocampal functions. Given that stimulus-enriched environments^101- 102 and physical activity^103- 105 potently enhance DG neurogenesis, the anterior hippocampal hypertrophy that we detected conceivably could also be a neuronal consequence of exaggerated stimulus-seeking behaviors in the children with ADHD. Moreover, stimulus seeking and attention to nontemporal stimuli are hypothesized to serve as strategies that reduce the length of experienced time while children with ADHD are experiencing the delayed delivery of an anticipated reward,⁹⁴ an experience to which they have an intense aversion.⁸ Thus, both stimulus seeking and its presumed morphological consequence, plastic hypertrophy in the anterior hippocampus, could help to allay disturbances in the perception of time and difficulties with delay aversion in children with ADHD.

The hippocampus is also the pacemaker for theta wave activity in the central nervous system,¹⁰⁶ and individuals with ADHD have unusually high relative theta activity (4-8 Hz) in their electroencephalograms.^107- 109 Thus, an enlarged hippocampus could account for excess theta activity in this group, particularly given that theta activity underlies working memory processes and the retrieval and consolidation of long-term memories^110- 112 and has been documented in animals during exploratory behaviors in unfamiliar surroundings.^113- 115

Although overall volume of the amygdala did not differ between subjects with ADHD and controls, surface analyses indicated the presence of significant reductions in volume overlying the lateral and basal nuclei, which together with the accessory basal nucleus have been designated the basolateral complex,¹¹⁶ a portion of the amygdala that is particularly densely connected with the PFC.^33,117- 118 Hyperactivity scores showed a trend toward positive correlation with overall volume of the amygdala and should as a statistical trend be interpreted cautiously. Nevertheless, surface analyses also detected positive correlations of hyperactivity symptoms with amygdala morphology at considerably greater levels of statistical significance, particularly in the region overlying the basolateral complex bilaterally, where volumes were reduced locally in the ADHD group. Inattention scores correlated inversely with surface measures most prominently over the basal and lateral nuclei of the left amygdala. Volume reductions and correlations with measures of symptom severity were localized primarily over the basolateral complex, the portion of the amygdala most consistently implicated in the attribution of affective valence to sensory stimuli,^119- 121 and the nuclei most likely to subserve fear conditioning.^122- 125 We postulate that morphological disturbances in the basolateral complex may interfere with both the attribution of valence to sensory stimuli and the development of normal fear responses in children with ADHD, which may in turn disrupt emotional learning and the affective drive to sustain attention to otherwise mundane sensory stimuli.

Interregional correlations suggested the presence of disturbed connectivity between the amygdala and OFC in the children with ADHD. The significant positive correlation of amygdalar volumes bilaterally with volumes of OFC gray matter in healthy controls was inverted significantly in the ADHD group. Connections between these regions are rich,^126- 127 and they support decision making by supplying information about positive and negative outcomes during choice behaviors.^36- 37 Neurons in the amygdala are thought to signal the value of specific reinforcers, information that is used subsequently by OFC neurons firing in expectation of the behavioral outcome to guide and reinforce behavior.¹²⁸ Interaction of the OFC and amygdala is therefore needed to learn reinforcements and to suppress unwanted behaviors,¹²⁹ as well as to evaluate the emotional and reinforcing salience of sensory stimuli.^32,130- 132 The poor performance of children with ADHD on delay-aversion tasks,⁸ their preferences for smaller immediate rewards,¹³³ and their more frequent risk-taking behaviors¹³⁴ all suggest that they are impaired in decision-making capabilities.¹³⁵ More generally, learning and behavioral control depend on the integrity of limbic-prefrontal connections, and we suspect that disturbances in these connections contribute to the impulsive behaviors that are a defining hallmark of ADHD.^13,136

The basolateral complex of the amygdala, in concert with the hippocampus and medial PFC, plays a central role in the consolidation of learning and memory functions, a role mediated through adrenergic, dopaminergic, and cholinergic neurotransmitter systems.^137- 138 Disruption of connectivity in amygdala-PFC pathways in children with ADHD is therefore consistent with some of the cognitive deficits associated with the disorder and with the cognition-enhancing effects of stimulant medications, which potentiate noradrenergic and dopaminergic transmission.¹³⁹

Two previous studies have reported normal hippocampal volumes in children and adolescents with ADHD. In both studies, 1 comprising 57²⁶ and the other 15 boys with ADHD,²⁷ larger hippocampal volumes were detected in the ADHD group, though not at the level of statistical significance. The statistical significance of our hippocampal findings may be attributable to a large sample size and to the use of images with higher resolution and improved signal-to-noise characteristics. Moreover, neither of the prior studies conducted detailed surface analyses of the hippocampus, which in our analyses revealed larger anterior and smaller posterior regions, effects that tend to offset one another when comparing overall volumes across diagnostic groups. These opposing effects within the same structure may explain why morphological abnormalities were difficult to detect previously.

The ultrastructural determinants of group differences in morphology of the hippocampus and amygdala are unknown, as is the extent to which disturbances in surface morphology relate to abnormalities in the underlying nuclei within these structures. Addressing these limitations will require detailed post-mortem studies. Additionally, the multiple statistical tests performed in our analyses increased the likelihood of type I error, which we minimized in our surface-based analyses through use of conservative statistical thresholds and GRF-based corrections for multiple comparisons.^62,140 Voxels that did not survive GRF correction of course should be interpreted with caution. Furthermore, correlations of surface morphology with clinical symptoms were exploratory and hypothesis generating and therefore also should be interpreted cautiously, as well as confirmed in future studies. Finally, we cannot entirely discount the possibility that medications or comorbid affective and anxiety disorders contributed to our findings, although we did not detect any evidence for these effects.

Our findings of hippocampal enlargement in children with ADHD and the association of progressively fewer symptoms with an increasing degree of this morphological abnormality suggest that hippocampal enlargement may represent neural responses within the hippocampus that compensate for problems in temporal processing and delay aversion. Disturbances in connectivity between the amygdala and OFC may contribute to problems of self-regulatory control and goal-directed behaviors. This study provides further evidence that the pathophysiology of ADHD involves limbic structures and limbic-prefrontal circuits.

Correspondence: Bradley S. Peterson, MD, Columbia University and the New York State Psychiatric Institute, 1051 Riverside Dr, Unit 74, New York, NY 10032 (petersob@childpsych.columbia.edu).

Submitted for Publication: June 14, 2005; final revision received December 29, 2005; accepted January 6, 2006.

Funding/Support: This work was supported in part by National Institute of Mental Health grants MHK02-74677, MH59139, and MH068318; grants from the National Alliance for Research in Schizophrenia and Affective Disorders; the Thomas D. Klingenstein & Nancy D. Perlman Family Fund; the Suzanne Crosby Murphy Endowment at Columbia University; and the Center for Child and Adolescent Mental Health, University of Bergen, Bergen, Norway.

Acknowledgment: We thank Xuejun Hao, PhD, and Ning Dong, MASc, for their technical assistance.