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Original Article |

Effect of Alzheimer Disease Risk on Brain Function During Self-appraisal in Healthy Middle-aged Adults FREE

Sterling C. Johnson, PhD; Michele L. Ries, PhD; Timothy M. Hess, PhD; Cynthia M. Carlsson, MD; Carey E. Gleason, PhD; Andrew L. Alexander, PhD; Howard A. Rowley, MD; Sanjay Asthana, MD; Mark A. Sager, MD
[+] Author Affiliations

Author Affiliations: Geriatric Research Education and Clinical Center, William S. Middleton Memorial Veterans Hospital (Drs Johnson, Ries, Hess, Carlsson, Gleason, and Asthana) and University of Wisconsin School of Medicine and Public Health, Departments of Medicine (Drs Johnson, Ries, Hess, Carlsson, Gleason, Asthana, and Sager), Medical Physics (Dr Alexander), and Radiology (Dr Rowley) Madison.


Arch Gen Psychiatry. 2007;64(10):1163-1171. doi:10.1001/archpsyc.64.10.1163.
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Context  Asymptomatic middle-aged adult children of patients with Alzheimer disease (AD) recently were found to exhibit functional magnetic resonance imaging (fMRI) deficits in the mesial temporal lobe during an encoding task. Whether this effect will be observed on other fMRI tasks is yet unknown. This study examines the neural substrates of self-appraisal (SA) in persons at risk for AD. Accurate appraisal of deficits is a problem for many patients with AD, and prior fMRI studies of healthy young adults indicate that brain areas vulnerable to AD such as the anterior mesial temporal lobe and posterior cingulate are involved during SA tasks.

Objective  To determine whether parental family history of AD (hereafter referred to as FH) or presence of the ε4 allele of the apolipoprotein E gene (APOE4) exerts independent effects on brain function during SA.

Design  Cross-sectional factorial design in which APOE4 status (present vs absent) was one factor and FH was the other. All participants received cognitive testing, genotyping, and an fMRI task that required subjective SA decisions regarding trait adjective words in comparison with semantic decisions about the same words.

Setting  An academic medical center with a research-dedicated 3.0-T MR imaging facility.

Participants  Cognitively normal middle-aged adults (n = 110), 51 with an FH and 59 without an FH.

Main Outcome Measure  Blood oxygen–dependent contrast measured using T2*-weighted echo-planar imaging.

Results  Parental family history of AD and APOE4 status interacted in the posterior cingulate and left superior and medial frontal regions. There were main effects of FH (FH negative > FH positive) in the left hippocampus and ventral posterior cingulate. There were no main effects of APOE genotype.

Conclusions  Our results suggest that FH may affect brain function during subjective SA in regions commonly affected by AD. Although the participants in this study were asymptomatic and middle-aged, the findings suggest that there may be subtle alterations in brain function attributable to AD risk factors.

Figures in this Article

Neuropathological studies14 of persons at risk for Alzheimer disease (AD) suggest that AD may be preceded by a silent preclinical phase in which the brain incurs neuropathological change. Presence of the ε4 allele of the apolipoprotein E gene (APOE4) and parental family history of AD (hereafter referred to as FH)5 are risk factors for developing late-onset AD.6 Identifying initial brain changes in at-risk individuals using noninvasive functional imaging may help elucidate preclinical presence and progression of early AD.

Most prior functional magnetic resonance imaging (fMRI) studies715 in populations at risk for AD have focused on the hippocampus using episodic memory tasks that draw on encoding or retrieval processes because memory symptoms are among the earliest to occur in AD. It has recently been shown that asymptomatic middle-aged adults (mean age, 55 years) who have a parent with AD exhibit reduced blood oxygenation level–dependent (BOLD) responses in the mesial and ventral temporal lobes during an encoding task,12 and this effect was not explained by APOE genotype. The findings suggest that memory-related brain changes attributable to FH may be occurring in regions vulnerable to AD approximately 2 decades before the typical age at onset in sporadic AD.

Other early symptoms of AD may involve high-level executive functions such as metacognitive appraisal. Studies on executive functions have often operationalized the construct to the cognitive control processes that subserve executive function, including working memory, mental flexibility and speed, imperviousness to distraction, and inhibition of prepotent responses. These well-studied functions have generally been attributed to the dorsolateral prefrontal cortex.16 The metacognitive aspects of executive function are less well studied and include processes such as self-monitoring, planning prospective action, making inferential or subjective decisions, self-appraisal (SA) (the focus of the present study), and social tuning of one's behavior for adaptive functioning in the world of people (judgment).

Prior studies have implicated cortical midline structures, including the anterior medial prefrontal cortex and posterior cingulate for metacognitive processes,1719 particularly the processes of self-regulation of affect, self-reflection on abilities and traits, self-monitoring of actions and bodily states, and social cognition such as making inferences about the mental states of others.20 The hippocampus has also been implicated in cognitive SA.21 Neuroimaging studies of the posterior cingulate have found a decreased cerebral metabolic rate of glucose metabolism,2224 cerebral atrophy,22,25 and amyloid binding22,26 in persons with AD. In 2 studies,4,24 subjects at risk for AD also exhibited reductions in cerebral metabolic rate of glucose in the posterior cingulate. Furthermore, “resting state” abnormalities in AD have been found in the posterior cingulate and medial frontal lobes, as well as the hippocampus.22,27,28

No studies have yet examined metacognitive brain function in healthy persons at risk for AD, to our knowledge. This may be a useful avenue of study because patients with AD often exhibit deficits in metacognitive abilities29 such as appreciating the extent or severity of their deficits. The objective of the present study was to determine whether we could observe AD risk–associated differences in BOLD activity during a self-referential decision task that consistently evokes BOLD activity from the posterior cingulate, medial frontal lobe, and mesial temporal lobes across prior studies19,3032 of healthy adults. We examined brain activation in 110 physically and cognitively asymptomatic middle-aged adults who differed in FH and APOE4 status. A 2 × 2 factorial design was used to examine the relative contribution of APOE genotype and FH on the cerebral response. Based on prior findings, we expected that FH would have an effect on cerebral activation that was separable from APOE genotype in cortical midline brain regions and hippocampus.

PARTICIPANTS

One hundred ten subjects underwent fMRI and cognitive testing (Table 1). Fifty-one (mean ± SD age, 53.6 ± 6.4 years) had at least 1 parent with AD (FH positive) and were recruited from the Wisconsin Registry for Alzheimer's Prevention,33 a longitudinal registry of cognitively normal adults between the ages of 40 and 65 years (at enrollment) who have at least 1 parent with sporadic AD. To verify the diagnosis of AD in the parent, parental medical records were obtained and were reviewed by a multidisciplinary diagnostic consensus panel. Typically, the clinical workup and diagnosis in the parent were conducted at the University of Wisconsin Memory Clinics, and the adult children were then approached for participation. The mean age at symptom onset in the affected parent was 73 years. All subjects in the FH-positive group underwent baseline neuropsychological evaluations and laboratory tests that included APOE genotyping using polymerase chain reaction and sequencing. Fifty-three percent (27 of 51) were ε4 positive (ε3/ε3, 24 subjects; ε3/ε4, 20 subjects; and ε4/ε4, 7 subjects).

Table Graphic Jump LocationTable 1. Demographic Neuropsychological and Performance Data for Each Groupa

Fifty-nine participants (mean ± SD age, 55.3 ± 6.2 years) with no FH (FH negative) were recruited from the community and were matched to the demographic characteristics of the FH-positive sample. Absence of FH was determined through self-report of the participant by telephone interview and by detailed medical history questionnaire. To be included in the FH-negative group, both parents had to survive to at least age 70 years (most were well beyond this age) and not have a diagnosis of dementia or exhibit frank symptoms of dementia of any kind. Twelve control subjects (20%) were ε4 positive (ε3/ε4, 11 subjects; and ε4/ε4, 1 subject), and 47 were ε4 negative (all ε3/ε3).

The demographics of the ε4-positive and ε4-negative subgroups are given in Table 1, along with neuropsychological and fMRI task performance. We only included participants who had the ε4 or ε3 allele of APOE (21 participants with ε2 alleles were excluded). This was done to control for potential heterogeneity among genotypes. The proportion of women in the cells differed; therefore, sex was used as a covariate in the fMRI data analysis. Exclusions for this imaging study included MR imaging scanner incompatibility, abnormal structural MR imaging or neuropsychological test results as part of study participation, or history of major psychiatric disease (eg, schizophrenia, substance dependence, and current or recent major depression) or major medical conditions (eg, type 1 diabetes mellitus, cancer requiring chemotherapy or radiation therapy, and neurological disorders, including prior head trauma with loss of consciousness). Most patients taking psychoactive drugs were excluded, although we allowed low-dose selective serotonin reuptake inhibitors if the patient had been stable for more than 3 months.

All subjects in the study received an additional fMRI task of episodic encoding that has been reported on previously.12 Participation in this study was contingent on signed informed consent, and the study was conducted in accord with the Declaration of Helsinki.

fMRI TASK

The fMRI paradigm has been described in detail in prior studies of healthy young adults30 and subjects with mild cognitive impairment (MCI).34 Briefly, the task requires participants to make yes or no decisions based on individually presented trait adjectives across 2 conditions, referential SA and nonreferential affective or semantic decision (SEM). The same set of 30 adjectives was presented in the SA and SEM conditions in counterbalanced order. In the SA condition, participants decided whether or not adjectives described their personal traits and abilities, whereas in the SEM condition, they decided whether or not adjectives in the set were of positive valence. The first presentation of each adjective was counterbalanced across conditions such that novelty was not confounded with condition order. Two equivalent forms of the task were presented sequentially (counterbalanced), each using separate adjective sets. Within each task run, each of the 2 conditions was presented in 5 pseudorandomized cycles. Words were presented every 4 seconds (3 seconds on screen and a 1-second interstimulus interval) in blocks of 6 per condition.

IMAGING PROCEDURES

After higher-order shimming, T2*-weighted gradient-echo echo-planar images were obtained as follows: echo time, 30 milliseconds; repetition time (TR), 2000 milliseconds; flip angle, 90°; acquisition matrix, 64 × 64 voxels; and field of view, 240 mm. Thirty sagittal sections of the brain were acquired within the TR at each time point, with voxel resolution of 3.75 × 3.75 × 4 mm and 1-mm skip between sections. In 4-minute and 8-second imaging trials, 124 time points were collected, of which 3 images acquired during the first 6 seconds were discarded (for a total of 242 reconstructed time points).

Residual magnetic field inhomogeneity resulting in regional distortions are common with echo-planar images. We corrected these by measuring 3-dimensional field maps across the brain (coplanar with the fMRI sections). This was accomplished by measuring the phase of non–echo-planar image gradient-echo images at 2 echo times (7 milliseconds and 10 milliseconds). The phase difference between the 2 echo images is proportional to the static field inhomogeneity.35 The warp calculation and correction36 were performed using the FMRIB Software Library version FSL3.2 (http://www.fmrib.ox.ac.uk/fsl). Anatomical T1-weighted volumes and T2-weighted axial seconds were also acquired using parameters previously described.12

For anatomical imaging and voxel-based morphometry analysis, axial T1-weighted and T2-weighted images were acquired after the functional runs. A 3-dimensional inversion recovery– prepared fast gradient echo–pulse sequence provided high-resolution T1-weighted structural images with the following parameters: inversion time, 600 milliseconds, fast gradient echo–pulse readout with TR/echo time/flip angle, 9 milliseconds/1.8 milliseconds/20°; acquisition matrix, 256 × 192 × 124 voxels (interpolated to 256 × 256 × 124 voxels); field of view, 240 mm; section thickness, 1.2 mm (124 sections); and ±160-kHz receiver bandwidth.

A fast-recovery fast spin-echo 2-dimensional T2-weighted axial sequence was also acquired with the same start and stop locations as the T1-weighted images. The parameters were as follows: field of view, 240 mm; acquisition matrix, 256 × 256 voxels; TR, 9000 milliseconds; echo time, 93 milliseconds; and flip angle, 90°. Seventy sections were acquired; the section thickness was 1.7 mm with a 0.3-mm skip. An experienced neuroradiologist (H.A.R.) examined all of the images before the analysis for clinical evidence of any neurovascular disease or structural abnormality that would exclude the subjects from the analysis.

DATA ANALYSIS

Other preprocessing steps and statistical analysis were performed using Statistical Parametric Mapping SPM2 software (http://www.fil.ion.ucl.ac.uk/spm). The image time series was motion corrected, field map corrected as already described, normalized into the Montreal Neurological Institute standard space using the T2-weighted template provided through SPM2 (written out with 2 × 2 × 2-mm voxel resolution), and then smoothed with an 8-mm full-width-at-half-maximum gaussian kernel.

The time-series data for individual participants were analyzed using a boxcar model convolved with the canonical hemodynamic response function as implemented in SPM2.37 The statistical model included high-frequency signal filtering (high-pass filter, 128 seconds) and the autoregressive(1) method of estimating temporal autocorrelation. The SA vs SEM contrast was computed for each participant and was entered into second-level analyses.

The mean effect of task (SA vs SEM), collapsed across groups, was first computed and thresholded at P < .005 uncorrected, which corresponded to Pfalse discovery rate–corrected < .04. This map was written out (Figure 1 and Table 2) and was used to constrain the subsequent analyses of group differences to only those brain voxels that were relevant to the task. Using analysis of covariance (ANCOVA), a 2 × 2 factorial analysis was performed that examined between-group effects of FH, APOE genotype, and FH × APOE genotype interaction. Sex was used as a covariate. This same design was applied to the demographic and neuropsychological data. For the fMRI factorial analyses, statistical significance was also assessed at a voxel-level threshold of P < .005 uncorrected.

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Figure 1.

Main effect of task across all 110 cognitively normal participants. Orthographic coronal, sagittal, and axial views are shown, as well as lateral and transverse maximum-intensity projections of the result. Left is on the left. The map is thresholded at Pfalse discovery rate < .05, corresponding to t > 2.57. The main effect of task in this comparison was used to restrict inference on subsequent comparisons of risk status. See Table 2 for cluster locations and statistical details. L indicates left; R, right. The scale bar from 0 to 14 represents the value of the t statistic.

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Table Graphic Jump LocationTable 2. Mean Effect of the Appraisal Task

For anatomical analyses, to determine whether group activation differences were due to anatomical differences in gray matter volume, we conducted voxel-based morphometry in the same search space and using the same model as the fMRI ANCOVA but with the additional covariate of total gray matter volume. The procedures have been described in detail elsewhere.12,38,39

Demographic characteristics and task performance are given in Table 1. The χ2 statistic indicated that the proportion of men and women differed in 1 group; therefore, sex was used as a covariate in subsequent behavioral and fMRI ANCOVAs. Factorial ANCOVA of demographic and neuropsychological variables indicated no FH × APOE genotype interaction. Tests for main effects of APOE genotype and FH in these healthy asymptomatic subjects were also statistically nonsignificant except for Trail Making Test A, on which the ε4-negative subjects performed 3.7 seconds faster than the ε4-positive subjects. There were no differences in the fMRI behavioral data with regard to reaction time and response bias. The fMRI findings follow.

EFFECT OF TASK

The mean response to the task (SA > SEM) is shown in Figure 1, with statistics and Montreal Neurological Institute locations given in Table 2. Active regions included 2 prominent midline clusters; the posterior cluster spans the ventral the posterior cingulate cortex, and the retrosplenial cortex.40 The large anterior medial prefrontal cortex cluster spans the medial surface of the superior frontal gyrus and rostral anterior cingulate. Also, 2 large bilateral clusters were observed in the anterior mesial temporal lobe spanning the hippocampus and amygdala and extending contiguously to the ventral forebrain and basal ganglia and thalamus. All comparisons in the subsequent factorial ANCOVA analysis were constrained to only those regions that were active in Figure 1. This procedure reduced the search region to 9.3% of the original number of voxels in the common brain mask. This was implemented to reduce the potential vulnerability to false-positive errors and to ensure that subsequent results from group comparisons were interpretable with regard to the cognitive task.

EFFECT OF RISK FACTORS

Using factorial ANCOVA, an F test for FH × APOE genotype interaction yielded prefrontal clusters at voxel location −26, 36, 36 (F = 9.73, P =.002 uncorrected; 102 voxels) in the left superior frontal gyrus and at voxel location −10, 48, 2 (F = 9.71, P =.002 uncorrected; 26 voxels) in the left anterior cingulate. A third small cluster was found in the retrosplenial area of the posterior cingulate at voxel location 0, −50, 4 (F = 8.26, P =.005 uncorrected; 33 voxels). These clusters and associated plots of signal change are shown in Figure 2. Post hoc analyses were conducted, and statistically significant mean differences are indicated on the plots. Group differences were only statistically significant relative to the FH-negative ε4-positive group. The other 3 groups did not differ from each other.

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Figure 2.

The interaction between first-degree family history of AD (hereafter referred to as FH) and APOE4 (ε4) status. The 3 clusters that reached statistical significance are shown in sagittal and axial views. The corresponding plots depict the means for the 4 groups (error bars indicate 95% confidence intervals [CIs]) derived from the first eigenvariate of each subject across the entire cluster. *The mean differed statistically significantly from the FH-negative (FH) ε4-positive (ε4+) group (P < .005). The scale bar from 0 to 9 represents the value of the F statistic for the interaction. FH+ indicates FH-positive group; ε4

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The main effects of FH and APOE genotype were tested next. Because main effects are not readily interpretable in the presence of interactions, voxels that were identified as statistically significant in the interaction map of Figure 2 were not considered in the analysis of main effects (this was achieved using the “mask with other contrasts” option in SPM2). The main effect of FH negative greater than FH positive was statistically significant in the left hippocampus (at voxel location −16, −22, −14; t = 4.05, P < .001 uncorrected; 233 voxels) and the left ventral posterior cingulate (at voxel location −14, −66, 20; t = 3.33, P = .001; 50 voxels). These results are shown in Figure 3. No statistically significant results were found in the reverse comparison (FH positive > FH negative). The effect of APOE genotype was tested using the contrast ε4 positive greater than ε4-negative (and its reverse). Statistically significant voxels in the interaction were again excluded. The results revealed no statistically significant voxels in either contrast.

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Figure 3.

Statistical parametric map of the main effect of first-degree family history of AD (hereafter referred to as FH). The figure shows regions where the FH-negative (FH) groups activate more on average than the FH-positive (FH+) groups. Select brain sections are shown (left is on the left) depicting the result in the posterior cingulate and subiculum with corresponding plots of the mean signal change derived from the first eigenvariate across the cluster (error bars indicate 95% confidence intervals [CIs]). The scale bar from 0 to 4 represents the value of the t statistic.

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ANATOMICAL ANALYSIS

Using voxel-based morphometry, no group differences in gray matter volume were found. These results suggest that the fMRI findings were not attributable to atrophy.

This study examined the cerebral response during a metacognitive task, SA on trait adjectives. We used this task in persons at risk for AD because converging research has indicated that the regions normally responsive on this task seem to overlap with brain regions affected by AD.3032 Our analyses indicated differences in task-related activation associated with FH, as well as regions where APOE genotype and FH risk factors interacted. Parental family history of AD had the effect of diminishing the cerebral response in the ventral posterior cingulate and the left mesial temporal lobe. Although there were no main effects of APOE4 status, this risk factor interacted with FH in the left dorsolateral prefrontal cortex, anterior medial prefrontal cortex, and retrosplenial posterior cingulate; plots indicated that ε4 carriers who had no FH exhibited greater signal change to the task. The observed effects were not due to gray matter atrophy or global cognitive function.

The medial parietal cortex has been implicated in memory retrieval and recognition,22,41,42 as well as metacognitive appraisals.19,31,32,4346 Several recent studies report medial parietal hypometabolism47 or hypoperfusion48 in individuals with MCI, a diagnosis that confers considerable risk for developing AD. Longitudinal studies4951 also indicate that posterior cingulate metabolism and regional blood flow discriminate between individuals with MCI who soon develop AD and patients with MCI who remain stable. Reiman and colleagues4,24 found that the medial parietal lobe, including the posterior cingulate cortex and precuneus, was hypometabolic for glucose in cognitively normal APOE4 carriers relative to noncarriers (the effect of FH was not tested in these earlier studies). The medial parietal findings observed in the present study during a cognitive challenge seem to be generally consistent with these prior results and suggest that this region may be beginning to exhibit dysfunction in these asymptomatic middle-aged adults at risk for AD. As further evidence of this possibility, Ries et al34 studied amnestic patients with MCI using the same paradigm reported herein and ratings of anosognosia. A statistically significant positive correlation was found between insight and activation; subjects with MCI who exhibit diminished insight for their cognitive impairment also exhibit diminished responses in the posterior cingulate and mesial frontal lobe. The data by Ries et al34 and the findings in the present study suggest that risk factors for AD are affecting systems supporting metacognition, which may eventually become part of the symptom picture of AD.

Areas of the left medial temporal lobe were also differentially active in FH-negative subjects on this task. In a young adult sample, it was recently shown that this region of the hippocampus exhibits task-dependent functional connectivity with the anterior medial prefrontal cortex on this same task.30 The hippocampus and subiculum are densely connected to the medial frontal lobe in rhesus monkeys.52,53 Phillips et al21 include the hippocampus in the dorsal axis of an emotion appraisal model (also involving the dorsomedial and dorsolateral frontal lobe) that receives biasing self-relevant input from ventral structures, including the amygdala, nucleus accumbens, and ventral medial frontal lobe.17 Amyloid burden in the mesial temporal lobe has been found to be related to the degree of anosognosia in patients with AD.54 The role of the hippocampus in affective and cognitive appraisal and how this might relate to the symptom picture of AD is not completely understood and is deserving of much more study.

In this sample, APOE4 status and FH interacted (Figure 2), but there were no APOE4 main effects. The interaction was largely due to the finding that ε4-positive subjects but not FH-negative subjects exhibited the greatest activation. An intriguing study by Mondadori et al55 points out several salutary effects of APOE4 status on the brain in early life, and they present fMRI results of a memory encoding task indicating that young adult ε3/ε4 carriers exhibit hippocampal learning-related signal adaptation but that young noncarriers do not. There is still much to learn about the effect of APOE4 status across the life span, but in the context of the recent literature and the findings in the present study, it is likely that interactions are occurring between APOE4 status and age or other AD risk factors that manifest as a putative salutary effect early in life but as a deleterious effect later in life.

The results herein are consistent with those of a prior study12 of an episodic encoding task among most of these same subjects in which a robust effect of FH in the hippocampus and ventral temporal lobes during object encoding was found. That prior study also found that the FH-negative ε4-positive group again exhibited the greatest cerebral response in the hippocampus, while FH-positive ε4-positive subjects had the least. A similar finding was observed when subjects possessing the ε2 allele were removed.15

At least 2 other recent studies have reported FH effects. In a behavioral experiment of odor identification, it was found that siblings of patients with AD exhibited reduced accuracy relative to control subjects. This effect was more pronounced in siblings who were APOE4 positive.56 Using fMRI, Bassett et al7 examined FH and APOE genotype in a large sample (n = 195) and found that FH affected brain activation during a paired-associate encoding task but that APOE genotype did not.

There remains a fundamental issue regarding fMRI group differences in cognitively normal vs at-risk or cognitively impaired populations. Some studies have reported risk-associated8,5759 and disease-associated10,60 increases in cerebral activity, while other studies report decreases in cerebral response with increased risk12,14,55 or cognitive impairment.13,6163 Although there are many sources of noise and variability using fMRI, some possible reasons for these study differences are the following: (1) Increased task difficulty has the effect of increasing fMRI activation.64,65 (2) Regarding the choice of comparison condition from which BOLD signal change is measured, it has been shown that a resting low-level baseline (such as rest or crosshair fixation) and an active cognitively challenging baseline produce different results.66,67 (3) Finally, the choice of analysis methods and statistical model (eg, spatially normalizing to a standard space vs native space68 or counting of suprathreshold voxels within a region vs statistical parametric mapping) may cause difference results.10 Given the variability across studies, researchers who develop fMRI tasks for use in clinical and at-risk populations should adopt a task-specific psychometric approach to measuring brain activation. Such an approach might include parametrically varying difficulty, comparison with normative data,55 and characterization of tasks across larger samples and across a range of demographics (eg, age) and clinical parameters (eg, genes and cognitive status61).

In conclusion, these data suggest that FH may affect brain function many years before typical disease onset. The genetic and environmental factors that embody FH are still largely unknown, and further study is required. Our results highlight the idea that factors beyond APOE genotype contribute to AD and should be included when possible in studies of AD risk. Although memory dysfunction is a core feature of AD and is typically one of the first noticeable symptoms, these findings using a self-referential decision task suggest that brain areas underlying metacognitive functions may also show compromise in persons at risk and may correspond, in part, to the metacognitive deficits that are observed in symptomatic AD.

Correspondence: Sterling C. Johnson, PhD, Geriatric Research Education and Clinical Center, William S. Middleton Memorial Veterans Hospital, 2500 Overlook Terr (11G), Madison, WI 53705 (scj@medicine.wisc.edu).

Submitted for Publication: October 2, 2006; final revision received March 19, 2007; accepted March 21, 2007.

Author Contributions: Dr Johnson had full access to all of the data in the study and takes responsibility for the integrity of the data and the accuracy of the data analysis.

Financial Disclosure: None reported.

Funding/Support: This study was supported by grant R01 AG21155 (Dr Johnson) and a merit review grant from the Department of Veterans Affairs.

Additional Contributions: Britta Jabbar, BS, Shelly Fitzgerald, BS, Gemma Gliori, MS, Lisa Newman, BS, Allison Wichmann, BS, Taylor Schmitz, BS, Mehul Trivedi, PhD, Michael Anderle, BS, and Ron Fisher, BS, provided additional assistance.

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PubMed
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PubMed
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PubMed
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PubMed
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PubMed
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PubMed
Alexander  GEChen  KPietrini  PRapoport  SIReiman  EM Longitudinal PET evaluation of cerebral metabolic decline in dementia: a potential outcome measure in Alzheimer's disease treatment studies. Am J Psychiatry 2002;159 (5) 738- 745
PubMed
Reiman  EMCaselli  RJYun  LSChen  KBandy  DMinoshima  SThibodeau  SNOsborne  D Preclinical evidence of Alzheimer's disease in persons homozygous for the ε4 allele for apolipoprotein E. N Engl J Med 1996;334 (12) 752- 758
PubMed
Scahill  RISchott  JMStevens  JMRossor  MNFox  NC Mapping the evolution of regional atrophy in Alzheimer's disease: unbiased analysis of fluid-registered serial MRI. Proc Natl Acad Sci U S A 2002;99 (7) 4703- 4707
PubMed
Price  JCKlunk  WELopresti  BJLu  XHoge  JAZiolko  SKHolt  DPMeltzer  CCDeKosky  STMathis  CA Kinetic modeling of amyloid binding in humans using PET imaging and Pittsburgh Compound-B. J Cereb Blood Flow Metab 2005;25 (11) 1528- 1547
PubMed
Lustig  CSnyder  AZBhakta  MO’Brien  KCMcAvoy  MRaichle  MEMorris  JCBuckner  RL Functional deactivations: change with age and dementia of the Alzheimer type [published online ahead of print November 7, 2003]. Proc Natl Acad Sci U S A 2003;100 (24) 14504- 1450910.1073/pnas.2235925100
PubMed
Greicius  MDSrivastava  GReiss  ALMenon  V Default-mode network activity distinguishes Alzheimer's disease from healthy aging: evidence from functional MRI [published online ahead of print March 15, 2004]. Proc Natl Acad Sci U S A 2004;101 (13) 4637- 464210.1073/pnas.0308627101
PubMed
Barrett  AMEslinger  PJBallentine  NHHeilman  KM Unawareness of cognitive deficit (cognitive anosognosia) in probable AD and control subjects. Neurology 2005;64 (4) 693- 699
PubMed
Schmitz  TWJohnson  SC Self-appraisal decisions evoke dissociated dorsal-ventral aMPFC networks [published online ahead of print December 2, 2005]. Neuroimage 2006;30 (3) 1050- 105810.1016/j.neuroimage.2005.10.030
PubMed
Johnson  SCBaxter  LCWilder  LSPipe  JGHeiserman  JEPrigatano  GP Neural correlates of self-reflection. Brain 2002;125 (pt 8) 1808- 1814
PubMed
Schmitz  TWKawahara-Baccus  TNJohnson  SC Metacognitive evaluation, self-relevance, and the right prefrontal cortex. Neuroimage 2004;22 (2) 941- 947
PubMed
Sager  MAHermann  BLa Rue  A Middle-aged children of persons with Alzheimer's disease: APOE genotypes and cognitive function in the Wisconsin Registry for Alzheimer's Prevention. J Geriatr Psychiatry Neurol 2005;18 (4) 245- 249
PubMed
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PubMed
Jezzard  PBalaban  RS Correction for geometric distortion in echo planar images from Bo field variations. Magn Reson Med 1995;34 (1) 65- 73
PubMed
Jenkinson  M Fast, automated, N-dimensional phase-unwrapping algorithm. Magn Reson Med 2003;49 (1) 193- 197
PubMed
Friston  KJ Commentary and opinion, II: statistical parametric mapping: ontology and current issues. J Cereb Blood Flow Metab 1995;15 (3) 361- 370
PubMed
Trivedi  MWichmann  AJabbar  BWard  MASchmitz  TWRies  MLKoscik  RLAsthana  SJohnson  SC Structural MRI discriminates individuals with mild cognitive impairment from age-matched controls: a combined neuropsychological and voxel based morphometry study. Alzheimers Dement 2007;2296- 302
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Vogt  BAVogt  LLaureys  S Cytology and functionally correlated circuits of human posterior cingulate areas [published online ahead of print September 6, 2005]. Neuroimage 2006;29 (2) 452- 46610.1016/j.neuroimage.2005.07.048
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Wagner  ADShannon  BJKahn  IBuckner  RL Parietal lobe contributions to episodic memory retrieval. Trends Cogn Sci 2005;9 (9) 445- 453
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Johnson  SCSchmitz  TWKawahara-Baccus  TNRowley  HAAlexander  ALLee  JDavidson  RJ The cerebral response during subjective choice with and without self-reference. J Cogn Neurosci 2005;17 (12) 1897- 1906
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Lane  RDFink  GRChau  PMDolan  RJ Neural activation during selective attention to subjective emotional responses. Neuroreport 1997;8 (18) 3969- 3972
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Marshall  GAKaufer  DILopez  OLRao  GRHamilton  RLDeKosky  ST Right prosubiculum amyloid plaque density correlates with anosognosia in Alzheimer's disease. J Neurol Neurosurg Psychiatry 2004;75 (10) 1396- 1400
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Mondadori  CRde Quervain  DJBuchmann  AMustovic  HWollmer  MASchmidt  CFBoesiger  PHock  CNitsch  RMPapassotiropoulos  AHenke  K Better memory and neural efficiency in young apolipoprotein E ε4 carriers [published online ahead of print October 31, 2006]. Cereb Cortex 2007;17 (8) 1934- 194710.1093/cercor/bhl103
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PubMed
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PubMed
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PubMed
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PubMed
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PubMed

Figures

Place holder to copy figure label and caption
Figure 1.

Main effect of task across all 110 cognitively normal participants. Orthographic coronal, sagittal, and axial views are shown, as well as lateral and transverse maximum-intensity projections of the result. Left is on the left. The map is thresholded at Pfalse discovery rate < .05, corresponding to t > 2.57. The main effect of task in this comparison was used to restrict inference on subsequent comparisons of risk status. See Table 2 for cluster locations and statistical details. L indicates left; R, right. The scale bar from 0 to 14 represents the value of the t statistic.

Graphic Jump Location
Place holder to copy figure label and caption
Figure 2.

The interaction between first-degree family history of AD (hereafter referred to as FH) and APOE4 (ε4) status. The 3 clusters that reached statistical significance are shown in sagittal and axial views. The corresponding plots depict the means for the 4 groups (error bars indicate 95% confidence intervals [CIs]) derived from the first eigenvariate of each subject across the entire cluster. *The mean differed statistically significantly from the FH-negative (FH) ε4-positive (ε4+) group (P < .005). The scale bar from 0 to 9 represents the value of the F statistic for the interaction. FH+ indicates FH-positive group; ε4

Graphic Jump Location
Place holder to copy figure label and caption
Figure 3.

Statistical parametric map of the main effect of first-degree family history of AD (hereafter referred to as FH). The figure shows regions where the FH-negative (FH) groups activate more on average than the FH-positive (FH+) groups. Select brain sections are shown (left is on the left) depicting the result in the posterior cingulate and subiculum with corresponding plots of the mean signal change derived from the first eigenvariate across the cluster (error bars indicate 95% confidence intervals [CIs]). The scale bar from 0 to 4 represents the value of the t statistic.

Graphic Jump Location

Tables

Table Graphic Jump LocationTable 1. Demographic Neuropsychological and Performance Data for Each Groupa
Table Graphic Jump LocationTable 2. Mean Effect of the Appraisal Task

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Alexander  GEChen  KPietrini  PRapoport  SIReiman  EM Longitudinal PET evaluation of cerebral metabolic decline in dementia: a potential outcome measure in Alzheimer's disease treatment studies. Am J Psychiatry 2002;159 (5) 738- 745
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PubMed
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PubMed
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PubMed
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PubMed
Johnson  SCBaxter  LCWilder  LSPipe  JGHeiserman  JEPrigatano  GP Neural correlates of self-reflection. Brain 2002;125 (pt 8) 1808- 1814
PubMed
Schmitz  TWKawahara-Baccus  TNJohnson  SC Metacognitive evaluation, self-relevance, and the right prefrontal cortex. Neuroimage 2004;22 (2) 941- 947
PubMed
Sager  MAHermann  BLa Rue  A Middle-aged children of persons with Alzheimer's disease: APOE genotypes and cognitive function in the Wisconsin Registry for Alzheimer's Prevention. J Geriatr Psychiatry Neurol 2005;18 (4) 245- 249
PubMed
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PubMed
Jezzard  PBalaban  RS Correction for geometric distortion in echo planar images from Bo field variations. Magn Reson Med 1995;34 (1) 65- 73
PubMed
Jenkinson  M Fast, automated, N-dimensional phase-unwrapping algorithm. Magn Reson Med 2003;49 (1) 193- 197
PubMed
Friston  KJ Commentary and opinion, II: statistical parametric mapping: ontology and current issues. J Cereb Blood Flow Metab 1995;15 (3) 361- 370
PubMed
Trivedi  MWichmann  AJabbar  BWard  MASchmitz  TWRies  MLKoscik  RLAsthana  SJohnson  SC Structural MRI discriminates individuals with mild cognitive impairment from age-matched controls: a combined neuropsychological and voxel based morphometry study. Alzheimers Dement 2007;2296- 302
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PubMed
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PubMed
Valenstein  EBowers  DVerfaellie  MHeilman  KMDay  AWatson  RT Retrosplenial amnesia. Brain 1987;110 (pt 6) 1631- 1646
PubMed
Wagner  ADShannon  BJKahn  IBuckner  RL Parietal lobe contributions to episodic memory retrieval. Trends Cogn Sci 2005;9 (9) 445- 453
PubMed
Johnson  SCSchmitz  TWKawahara-Baccus  TNRowley  HAAlexander  ALLee  JDavidson  RJ The cerebral response during subjective choice with and without self-reference. J Cogn Neurosci 2005;17 (12) 1897- 1906
PubMed
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PubMed
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