One microgram of RNA was reverse transcribed into complementary DNA (cDNA) using the High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems). Reverse transcription was performed in an Eppendorf Mastercycler (Eppendorf, Hamburg, Germany) for 10 minutes at 25°C, 120 minutes at 37°C, and 5 seconds at 85°C. The TaqMan PreAmp Master Mix Kit (Applied Biosystems) was used to increase the quantity of specific cDNA targets for QPCR analysis. For the preamplification reaction, the primers and probes of 5 TaqMan assays (SST, PVALB, GAD1, ACTB, and FLNA) were pooled together, with 1× TRIS–EDTA buffer. The preamplification reaction was performed in 50-μL reactions containing 25 μL of TaqMan PreAmp Master Mix, 12.5 μL of the pooled assay mix, 10 μL of cDNA sample, and 2.5 μL of nuclease-free water. Cycling conditions were 10 minutes at 95°C, 14 cycles of 15 seconds at 95°C, and 4 minutes at 60°C using an Eppendorf Mastercycler.