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Dexamethasone Suppression Test: Technique and Accuracy

Victor S. Fang, PhD; Barbara Warenica, MD; Herbert Y. Meltzer, MD
Arch Gen Psychiatry. 1982;39(10):1217-1217. doi:10.1001/archpsyc.1982.04290100073012
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To the Editor.  —The dexamethasone suppression test (DST) is of great current interest in psychiatry because of the large body of evidence that some depressed patients fail to suppress cortisol levels to 5 μg/dL or less after an oral dose of 1.0 mg of dexamethasone.1 Many psychiatrists have adopted this procedure as the first laboratory test of significance for clinical practice. Carroll et al pointed out a number of pitfalls in applying this test.1 We wish to call attention to the need to assess rigorously the method of cortisol determination used by the clinical laboratories that carry out the test. Routine application of the 5-μg/dL cutoff could lead to a number of false-positive results, based on the results to be described.Research laboratories, eg, those of Carroll, Sachar, and ourselves, use the competitive protein-binding assay (CPBA) method to measure plasma cortisol levels, whereas most clinical laboratories use commercial radioimmunoassay (RIA)kits.

REFERENCES

Carroll BJ, Feinberg M, Greden JF, et al:  A specific laboratory test for the diagnosis of melancholia . Arch Gen Psychiatry 1981;;38:15-22.
Jekowsky E, Trainer TD, Copeland BE:  Comparison of an automated radioimmunoassay method and a competitive protein-binding method for evaluation of serum cortisol . Am J Clin Pathol 1979;;72:146-150.
Murphy BEP:  Some studies of the protein-binding of steroids and their application to the routine micro- and ultramicro-measurement of various steroids in body fluids by competitive protein-binding radioassay . J Clin Endocrinol Metabol 1967;;27:973-990.

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Carroll BJ, Feinberg M, Greden JF, et al:  A specific laboratory test for the diagnosis of melancholia . Arch Gen Psychiatry 1981;;38:15-22.
Jekowsky E, Trainer TD, Copeland BE:  Comparison of an automated radioimmunoassay method and a competitive protein-binding method for evaluation of serum cortisol . Am J Clin Pathol 1979;;72:146-150.
Murphy BEP:  Some studies of the protein-binding of steroids and their application to the routine micro- and ultramicro-measurement of various steroids in body fluids by competitive protein-binding radioassay . J Clin Endocrinol Metabol 1967;;27:973-990.

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